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中国临床药理学与治疗学 ›› 2017, Vol. 22 ›› Issue (7): 749-754.

• 基础研究 • 上一篇    下一篇

黄芩苷对哮喘大鼠气道重塑作用的实验研究

韩 超1,杨 柳2,张秋玲1,潘竞锵1,徐 俊2   

  1. 1广州市中医医院,广州 510130,广东;2暨南大学药学院,广州 510632,广东
  • 收稿日期:2016-12-22 修回日期:2017-02-08 出版日期:2017-07-26 发布日期:2017-07-19
  • 作者简介:韩超,女,本科,副主任药师,研究方向:中药药理学。 Tel: 020-81226220 E-mail: wendyhan@163.com
  • 基金资助:

    广东省中医药局基金项目(20141226);广州市中医药、中西医结合项目(20142A011001)

Effect of baicalin on airway remodeling of bronchial asthma

HAN Chao 1, YANG Liu 2, ZHANG Qiuling 1, PAN Jinqiang 1, XU Jun 2   

  1. 1 Guangzhou Hospital of Chinese Medicine, Guangzhou 510130, Guangdong, China; 2 College of Pharmacy, Jinan University, Guangzhou 510632, Guangdong, China
  • Received:2016-12-22 Revised:2017-02-08 Online:2017-07-26 Published:2017-07-19

摘要:

目的: 初步探讨黄芩苷对支气管哮喘气道重塑的防治作用及其作用机制。方法: 用卵清白蛋白(OVA)致敏大鼠制备支气管哮喘气道重塑动物模型,经黄芩苷干预治疗,用免疫组化法检测各组大鼠肺组织匀浆中α-平滑肌肌动蛋白(α-SMA)、磷酸化细胞外信号调节激酶1/2(p-ERK1/2)及气道组织中转化生长因子-β1(TGF-β1)蛋白的表达量;用RT-PCR检测气道平滑肌(ASM)中p-ERK1/2的mRNA水平。结果: 实验结果显示,p-ERK1/2、α-SMA、TGF-β1蛋白的表达量在模型组中有明显的增加,地塞米松组、黄芩苷高剂量组和低剂量组的p-ERK1/2、α-SMA、TGF-β1蛋白的表达量均低于模型组(P<0.05,P<0.01)。结论: 黄芩苷可降低TGF-β1、α-SMA的蛋白表达,并影响ERK信号传导通路,下调p-ERK1/2 mRNA含量,从而减轻气道炎症,延缓气道重塑。

关键词: 黄芩苷, 气道重塑, ERK信号通路, TGF-β1, α-SMA

Abstract:

AIM: To explore the effect and mechanism of baicalin on the airway remodeling of bronchial asthma.  METHODS: Animal models of airway remodeling in asthmatic rats were induced by egg albumen (OVA). After the treatment of Baicalin, expressions of TGF-β1, p-ERK1/2 and α-SMA in the lung tissue of rats were detected by immunohistochemical method, and the level of p-ERK1/2 mRNA in the airway smooth muscle (ASM) was detected by RT-PCR. RESULTS:Expressions of p-ERK1/2, α-SMA and TGF-β1 were significantly increased in the model group, which were all higher than those of dexamethasone group, baicalin high dose group and low dose group (P<0.05 or P<0.01). CONCLUSION: Baicalin can decrease the expression of TGF-β1, α-SMA, affect the ERK signal pathways, down regulate the content of p-ERK1/2 mRNA , thereby reducing airway inflammation and delaying airway remodeling.

Key words: baicalin, airway remodeling, ERK signal pathways, TGF-β1, α-SMA

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