脑心通胶囊对自发性高血压大鼠肾损伤的保护作用

中国临床药理学与治疗学 ›› 2017, Vol. 22 ›› Issue (8): 875-880.

脑心通胶囊对自发性高血压大鼠肾损伤的保护作用

杨小虎¹，廉洪¹，余杨¹，岳影星²，张勇³

¹浙江医院药剂科，杭州 310013，浙江； ²浙江医院浙江省老年医学研究所，杭州 310013，浙江；³湖北医药学院药学院，十堰 442000，湖北

收稿日期:2017-03-07 修回日期:2017-04-03 出版日期:2017-08-26 发布日期:2017-08-18
通讯作者: 浙江中医药科学基金研究项目（2017ZB002）；浙江医院院级课题项目（2015YJ038）
作者简介:杨小虎，男，硕士，药师，主要从事心血管药理研究。 Tel：0571-81595229 E-mail：xtigeryang@yeah.net
基金资助:
浙江中医药科学基金研究项目（2017ZB002）；浙江医院院级课题项目（2015YJ038）

Protective effect of Naoxintong capsule on kidney injury in spontaneously hypertensive rats

YANG Xiaohu ¹, LIAN Hong¹, YU Yang ¹, YUE Yingxing ², ZHANG Yong³

¹Department of Pharmacy, Zhejiang Hospital, Hangzhou 310013, Zhejiang, China; ² Zhejiang Provincial Key Laboratory of Geriatrics & Geriatrics Institute, Zhejiang Hospital, Hangzhou 310013, Zhejiang, China; ³ College of Pharmacy, Hubei University of Medicine, Shiyan 442000, Hubei, China

Received:2017-03-07 Revised:2017-04-03 Online:2017-08-26 Published:2017-08-18

摘要/Abstract

摘要：

目的: 探讨脑心通胶囊（NXTC）对高血压大鼠肾损伤的保护作用。方法: 24只雄性自发性高血压大鼠（spontaneously hypertensive rats,SHR）随机分为模型（Model）组、厄贝沙坦（Irbesartan）组及NXTC组，8只雄性Wistar-Kyoto（WKY）大鼠为空白（Blank）组。各组大鼠分别给予相应药物和生理盐水灌胃8周，无创血压测量仪监测各组平均血压变化；生化分析试剂盒分别检测血清中肌酐（Cre）、尿酸（UA）、尿素（Urea）以及尿微量白蛋白（MALB）；苏木精-伊红（H&E）染色观察药物对肾组织病理学改变；实时定量RT-PCR检测肾组织间肌红蛋白（myoglobin,Mb）、Nox氧化酶1（NADPH oxidase 1,Nox1）以及Duox氧化酶2（dual oxidase 2,Duox2）基因表达变化；Western blot检测肾组织中Mb蛋白表达变化。结果: 与Model组相比，SHR大鼠连续灌胃给药NXTC 8周后，平均血压下降14.9%；大鼠血清中Cre、UA、MALB分别下降47.1%、20.8%及25.9%；组织病理学显示，NXTC组肾损伤低于模型组；qRT-PCR显示，NXTC组的Mb、Nox1及Duox2分别下调36.8%、26.2%以及30.2%。Western Blot显示，与Model组相比,NXTC组Mb蛋白表达与基因表达一致，呈正相关。结论: NXTC可改善SHR大鼠的肾损伤因子水平，下调肾组织Mb过表达，减轻肾损伤。

关键词: 脑心通胶囊, 高血压, 肾损伤, 肌红蛋白

Abstract:

AIM: To investigate the protective effect of Naoxintong capsule (NXTC) on kidney injury in spontaneously hypertensive rats. METHODS: Twenty-four female spontaneously hypertensive rats (SHR) were randomly divided into model group, Irbesartan group and NXTC group, while eight female normotensive Wistar-Kyoto rats (WKY) were blank group. Rats in each group were intragastricly administrated with corresponding drugs and saline in eight weeks. Non-invasive blood pressure (BP) measuring instrument monitored BP of each group rats in every two weeks. The serum levels of total creatinine (Cre), uric acid (UA), urea (Urea) and microalbumin (MALB) were measured. Kidney injury was evaluated with histopathologic examination by hematoxylin eosin (H&E) staining. Real-time RT-PCR was used to test the gene expression changes of Mb, Nox1 and Duox2 in each group. And the expression of Mb in kidney tissue was measured by Western blot. RESULTS:NXTC significantly decreased mean BP in SHR group. Serum levels of Cre, UA and MALB in NXTC group were significantly lower than those in the model group (All P<0.01). Kidney injure in NXTC group was decreased as compared with model group (P<0.01). NXTC significantly reversed not only the down-regulation expression of Mb, Nox1 and Duox2 genes (All P<0.01), but also Mb protein (P<0.01) in SHR group. CONCLUSION: NXTC can protect SHR rats from kidney injury at least partly via regulating Mb overexpression.

Key words: Naoxintong capsule, hypertension, kidney injury, Mb

中图分类号:

R965.2

杨小虎，廉洪，余杨，岳影星，张勇. 脑心通胶囊对自发性高血压大鼠肾损伤的保护作用[J]. 中国临床药理学与治疗学, 2017, 22(8): 875-880.

YANG Xiaohu, LIAN Hong, YU Yang, YUE Yingxing, ZHANG Yong. Protective effect of Naoxintong capsule on kidney injury in spontaneously hypertensive rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(8): 875-880.

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