乳复宁颗粒调节乳腺增生症家兔激素水平以及VEGF蛋白表达变化的研究

doi:10.12092/j.issn.1009-2501.2018.02.004

中国临床药理学与治疗学 ›› 2018, Vol. 23 ›› Issue (2): 138-142.doi: 10.12092/j.issn.1009-2501.2018.02.004

乳复宁颗粒调节乳腺增生症家兔激素水平以及VEGF蛋白表达变化的研究

李睿¹，孔艳²，刘增辉¹，罗胜勇¹

¹安徽省医学科学研究院，合肥 230061，安徽；²安徽医科大学解剖教研室，合肥 230061，安徽

收稿日期:2017-08-10 修回日期:2017-09-14 出版日期:2018-02-26 发布日期:2018-03-02
通讯作者: 罗胜勇，男，博士，副研究员，研究方向：心脑血管药理。 Tel: 13855137256 E-mail: lsy770728@126.com
作者简介:李睿，男，硕士，助理研究员，研究方向：心脑血管药理。 Tel: 13705606230 E-mail: liruianyi@163.com
基金资助:
安徽省自然科学基金面上项目（1608085MH161）

Effects of Rufuning granules on hormone levels and the expression of VEGF protein in mammary gland hyperplasia rabbits

LI Rui ¹，KONG Yan ²，LIU Zenghui ¹，LUO Shengyong ¹

¹ Anhui Academy of Medical Sciences, Hefei 230061, Anhui, China; ²Department of Anatomy, Anhui Medical University, Hefei 230061, Anhui, China

Received:2017-08-10 Revised:2017-09-14 Online:2018-02-26 Published:2018-03-02

摘要/Abstract

摘要：

目的: 研究乳复宁颗粒改善乳腺增生疾病症状的作用及机制。方法: 将48只家兔按体质量随机分为6组，每组8只，即正常对照组、模型对照组、乳癖消片组、乳复宁颗粒高、中、低剂量组。给予雌性未孕家兔注射苯甲酸雌二醇，建立乳腺增生病理模型，测定家兔乳房直径和乳头高度，并检测各组家兔血清中泌乳素（PRL）、雌二醇（E2）、孕酮（P）的含量，镜下观察乳腺组织病理形态学变化。同时测定家兔乳腺组织中血管内皮生长因子（VEGF）蛋白的表达改变。结果: 乳复宁颗粒高、中剂量组可降低乳腺增生症家兔乳房直径和乳头高度（P<0.01），降低血清中PRL、E2的含量（P<0.01），增加血清中P的含量；减少乳腺小叶数、小叶腺泡数，减轻管腔扩张和乳腺间质纤维结缔组织增生；同时降低乳腺组织中VEGF的过度表达。结论: 乳复宁颗粒可能通过调整雌孕激素比例及降低VEGF的表达来改善乳腺增生的症状。

关键词: 乳复宁颗粒, 乳腺增生, 雌二醇, 孕酮, VEGF蛋白

Abstract:

AIM: To investigate the mechanism and the effects of Rufuning granules on improving the symptom of mammary gland hyperplasia（MGH）． METHODS: Forty-eight rabbits were randomly divided into six groups (n=8), normal control group, model group, Rupixiao tablets group, Rufuning-high dose, medium dose and low dose groups. The model of MGH was induced by injecting estradiol benzoate in unpregnant female rabbits. Rabbits in the normal control received saline injection only. The breast diameter and nipple height of rabbits were determined. The contents of prolactin（PRL）, estradiol（E2）and progesterone（P）in serum in each group were detected．Pathologic changes in morphology due to MGH in rabbits were observed by microscopy. Meanwhile, the expression of VEGF protein in rabbit breast tissue was determined. RESULTS: The breast diameter and nipple height of rabbits were reduced after treatment in high and medium doses of Rufuning granules group（P<0.05）．The contents of PRL and E2 in serum were reduced（P<0.05）while those of progesterone in serum were improved（P<0.05）．The performance of histology breast hyperplasia was significantly improved. The number of mammary gland flocculus and lobular gland bubble and lumen expansion were reduced in rabbit mammary. Mammary gland hyperplasia of interstitial fibrous connective tissue was also significantly alleviated as compared with those in model group．Meanwhile, VEGF over-expression in breast tissue was reduced. CONCLUSION: Rufuning granules may improve symptom of MGH by adjusting the ratio of estrogen and progesterone and reducing VEGF over-expression．

Key words: Rufuning granules, mammary gland hyperplasia, estradiol, progesterone, VEGF

中图分类号:

R965.2

李睿，孔艳，刘增辉，罗胜勇. 乳复宁颗粒调节乳腺增生症家兔激素水平以及VEGF蛋白表达变化的研究[J]. 中国临床药理学与治疗学, 2018, 23(2): 138-142.

LI Rui，KONG Yan，LIU Zenghui，LUO Shengyong. Effects of Rufuning granules on hormone levels and the expression of VEGF protein in mammary gland hyperplasia rabbits[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(2): 138-142.

参考文献

［1］谷丽艳,易佳丽,樊延宏,等. 中医药疗法治疗乳腺增生研究进展［J］.辽宁中医药大学学报,2014,16（1）:173-176.
［2］Valerie C,Cathy S,Andrea Z,et al. PELP1 overexpression in the mouse mammary gland results in the development of hyperplasia and carcinoma［J］.Cancer Res,2014,74(24):7395-7405.
［3］Liu J,Esmailpour T,Shang XY,et al.TBX3 over-expression causes mammary gland hyperplasia and increases mammary stem-like cells in an inducible transgenic mouse model［J］.BMC Dev Biol,2011,11:65.
［4］章建民,陈宇,杨明艳,等. 乳腺增生兔模型的制备及初步评价消癖健乳巴布膏的药效［J］. 中华中医药学刊,2013,31（10）:2166-2168.
［5］Tian J, Lambertz I, Thomas R, et al. Transgenic insulin-like growth factor-1 stimulates activation of COX-2 signaling in mammary glands［J］. Mol Carcinog, 2012, 51(12): 973-983.
［6］Wang CS, Sun B, Guo L. Volatile organic metabolites identify patients with breast cancer, cyclomastopathy, and mammary gland fibroma［J］. Sci Rep,2014, 4: 5383.
［7］虞昆,倪超,宋向阳,等. 乳腺癌组织中CD73表达及其与临床病理特征的相关性研究［J］. 中国临床药理学与治疗学,2017,22（5）:551-555.
［8］Arendt LM, Kuperwasser C. Form and function: how estrogen and progesterone regulate the mammary epithelial hierarchy［J］. J Mammary Gland Biol Neoplasia,2015,20(1/2): 9-25.
［9］林中琳,董乃维.治疗乳腺癌小分子药物研究进展［J］.中国临床药理学与治疗学,2015,20（8）:956-960.
［10］Guo LY, Liu S, Jakulin A, et al. Positive expression of cyclin D1 is an indicator for the evaluation of the prognosis of breast cancer［J］. Int J Clin Exp Med,2015,8(10): 18656-18664.
［11］Howell A, Anderson AS, Clarke RB, et al. Risk determination and prevention of breast cancer［J］. Breast Cancer Res,2014,16（5）: 446.
［12］夏丽娟,何云芹,邵明君. 左炔诺孕酮宫内节育系统治疗无排卵型功能失调性子宫出血的疗效［J］.中国临床药理学与治疗学,2017,22（6）:699-704.
［13］Zhang YN, Liu J, Lin J, et al. The transcription factor GATA1 and the histone methyltransferase SET7 interact to promote VEGF-mediated angiogenesis and tumor growth and predict clinical outcome of breast cancer［J］. Oncotarget, 2016,7(9): 9859-9875.

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乳复宁颗粒调节乳腺增生症家兔激素水平以及VEGF蛋白表达变化的研究

Effects of Rufuning granules on hormone levels and the expression of VEGF protein in mammary gland hyperplasia rabbits

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