调节性B细胞在自身免疫性溶血性贫血患者外周血中的表达及临床意义

doi:10.12092/j.issn.1009-2501.2018.03.015

中国临床药理学与治疗学 ›› 2018, Vol. 23 ›› Issue (3): 329-332.doi: 10.12092/j.issn.1009-2501.2018.03.015

调节性B细胞在自身免疫性溶血性贫血患者外周血中的表达及临床意义

何合胜，靳小可，徐又海，杨玉琼，刘善浩，齐晶

皖南医学院第一附属医院血液内科，芜湖 241000，安徽

收稿日期:2017-12-12 修回日期:2018-01-20 出版日期:2018-03-26 发布日期:2018-03-28
作者简介:何合胜，男，硕士，副主任医师，研究方向：血液系统疾病的免疫机制研究。 E-mail：hhsmed2012@126.com
基金资助:
皖南医学院重点科研项目培育基金（WK2015ZF02）

Number of regulatory B cells in peripheral blood of patients with autoimmune hemolytic anemia and its clinical signifcance

HE Hesheng, JIN Xiaoke, XU Youhai, YANG Yuqiong, LIU Shanhao, QI Jing

Department of Hematology, the First Affiliated Hospital of Wannan Medical College, Wuhu 241000, Anhui, China

Received:2017-12-12 Revised:2018-01-20 Online:2018-03-26 Published:2018-03-28

摘要/Abstract

摘要：

目的: 探讨自身免疫性溶血性贫血（AIHA）患者外周血中调节性B细胞（Bregs）的表达及其在该病发病中的意义。方法:选择16例自身免疫性溶血性贫血患者和14例健康志愿者为研究对象，用流式细胞术分析外周血CD19+IL-10+调节性B细胞及CD19+CD24hiCD27+调节性B细胞的表达；ELISA方法检测培养上清液中IL-10的水平。结果:自身免疫性溶血性贫血患者外周血中CD19+IL-10+调节性B细胞、CD19+CD24hiCD27+调节性B细胞的表达分别为（1.27±0.39）％、（9.85±2.18）％，健康志愿者组分别为（2.92±0.71）％、（26.47±4.31）％，两组比较差异有统计学意义（P<0.05）；自身免疫性溶血性贫血患者细胞培养上清中IL-10的水平低于健康志愿者组（P<0.05）。结论:自身免疫性溶血性贫血患者外周血中CD19+IL-10+调节性B细胞及CD19+CD24hiCD27+调节性B细胞比例降低，提示调节性B细胞可能参与自身免疫性溶血性贫血的发病过程。

关键词: 自身免疫性溶血性贫血, 调节性B细胞, 白细胞介素-10

Abstract:

AIM: To explore the number of regulatory B cells (Bregs) in peripheral blood of patients with autoimmune hemolytic anemia (AIHA) and its significance in the incidence of AIHA. METHODS: A total of 16 AIHA patients and 14 healthy controls were enrolled. The expressions of CD19+IL-10+ Bregs and CD19+CD24hiCD27+ Bregs in peripheral blood mononuclear cells (PBMCs) were evaluated by flow cytometry. And levels of IL-10 were measured in the culture supermatants by ELISA. RESULTS: The expression of CD19+IL-10+ Bregs in PBMCs of AIHA patients was (1.27±0.39)%, which was lower than that in healthy controls (2.92±0.71)% (P<0.05). The number of CD19+CD24hiCD27+ Bregs in AIHA patients was (9.85±2.18)%, which was lower than that in healthy controls (26.47±4.31)% (P<0.05). The level of IL-10 significantly decreased in culture supermatants of autoimmune hemolytic anemia patient as compared with healthy controls (P<0.05). CONCLUSION: The expression of Bregs significantly decreases in PBMCs of AIHA patients. Bregs may play some role in the pathogenesis of AIHA.

Key words: autoimmune hemolytic anemia, regulatory B cells, interleukin-10

中图分类号:

R556.6

何合胜，靳小可，徐又海，杨玉琼，刘善浩，齐晶. 调节性B细胞在自身免疫性溶血性贫血患者外周血中的表达及临床意义[J]. 中国临床药理学与治疗学, 2018, 23(3): 329-332.

HE Hesheng, JIN Xiaoke, XU Youhai, YANG Yuqiong, LIU Shanhao, QI Jing. Number of regulatory B cells in peripheral blood of patients with autoimmune hemolytic anemia and its clinical signifcance[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(3): 329-332.

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调节性B细胞在自身免疫性溶血性贫血患者外周血中的表达及临床意义

Number of regulatory B cells in peripheral blood of patients with autoimmune hemolytic anemia and its clinical signifcance

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