关键词: 柴胡解毒汤, 尖锐湿疣, T淋巴细胞亚群, 自然杀伤细胞, 细胞因子

Abstract:

AIM: To observe the clinical efficacy of the Chaihu Jiedu Decoction in the treatment of condyloma acuminatum (CA), and to explore the possible effective mechanism. METHODS: A total of 120 patients with CA (age range from 16 to 65) who were admitted and treated in our hospital from June 2015 to September 2017 were selected. All patients were randomly divided into three groups: group A received Chaihu Jiedu Decoction（n=40）, group B received α-2b recombinant human interferon（n=39, one of the patients withdrew from the study due to adverse reactions after 2 weeks of medication）and group C received no systemic therapy（n=40）. The clinical efficacy, recurrence rate, recurrence frequency and adverse drug adverse reaction of the three groups were compared. Peripheral blood T lymphocyte subsets and NK cells before and after the treatment of CA patients in group A were detected by flow cytometry, and IL-2, IL-4, IL-10, IL-12, IFN-γ in peripheral venous blood serum were detected by ELISA. RESULTS:Except for the one withdrawal case from group B, after 12 weeks follow-up, the rate and the average times of recurrence in all of the CA cases were 51.26%（61/119）and 1.6, respectively. The rate and the average times of recurrence in group A and B were lower than those of group C（P<0.01）, but the differences between group A and B were not statistically significant（P>0.05）. At the end of one month treatment with Chaihu Jiedu Decoction, the CD4+T cell percentage, the CD4+/CD8+ cell ratio, and the NK cell percentage were all increased（P<0.01）in peripheral blood of CA patients, but the differences of CD3+T cell percentage and CD3+T cell percentage were not statistically significant. Also, at the end of treatment with Chaihu Jiedu Decoction, the peripheral blood serum IL-2 of CA patients increased（P<0.01）, the IL-12（P<0.01）, IFN-γ（P<0.01）and IL-10 decreased（P<0.05）, while the difference of IL-4 content was not statistically significant（P>0.05）. CONCLUSION:The Chaihu Jiedu Decoction has significant clinical effect on CA. The efficacy is comparable to intramuscular injection of α-2b recombinant human interferon. The effective mechanism may be the improvement of cell-mediated immunity function by promoting the number and function of CD4+T cell, and the increase of the number and function of NK cells by promoting the increase of cytokines Il-2 and IFN-γ secreted by CD4+T cells.

Key words: Chaihu Jiedu Decoction, condyloma acuminatum, T lymphocyte subsets, natural killer cell, cytokines