生长激素宫腔灌注治疗薄型子宫内膜的研究

doi:10.12092/j.issn.1009-2501.2019.09.004

中国临床药理学与治疗学 ›› 2019, Vol. 24 ›› Issue (9): 983-988.doi: 10.12092/j.issn.1009-2501.2019.09.004

生长激素宫腔灌注治疗薄型子宫内膜的研究

曹志文，胡卫华

皖南医学院第一附属医院弋矶山医学生殖中心，芜湖 241001，安徽

收稿日期:2019-04-26 修回日期:2019-07-15 出版日期:2019-09-26 发布日期:2019-09-26
通讯作者: 胡卫华，女，副教授，副主任医师，研究生导师，研究方向：生殖医学。 Tel：0553-5739341 E-mail：wnmchwh@163.com
作者简介:曹志文，男，硕士研究生在读，研究方向：生殖医学。 Tel：0553-5739341 E-mail：wysrnl@163.com
基金资助:
国家自然科学基金（21645006）；2018年弋矶山医院人才引进（KY25220349）；安徽省教育厅自然科学重点项目（KJ2016A724）

Effects of intrauterine perfusion of growth hormone on thin endometrium

CAO Zhiwen, HU Weihua

Reproductive Medicine Center, Yijishan Hospital of Wannan Medical College, Wuhu 241001, Anhui, China

Received:2019-04-26 Revised:2019-07-15 Online:2019-09-26 Published:2019-09-26

摘要/Abstract

摘要：

目的:研究生长激素（GH）宫腔灌注对子宫内膜过薄的治疗作用。方法:雌性SD大鼠30只，随机数字法分三组，对照组（n=10），模型组（n=10）及GH组（n=10）。对照组常规饲养。模型组和GH组在动情期通过95%乙醇宫腔内灌注构建薄型子宫内膜模型。建模后6～8 h，模型组进行宫腔灌注0.2 mL生理盐水，GH组宫腔灌注生长激素0.2 mL。宫腔治疗后阴道涂片3个动情期，于动情期处死大鼠取子宫，采用HE染色制片并于显微镜下观察子宫内膜厚度（μm），血管数目、腺体数量的变化。经过免疫印迹、免疫组织化学方法，检验大鼠子宫腺上皮细胞角蛋白以及间质细胞波形蛋白表达。结果:模型组与对照组内膜厚度比较相对减小（P<0.01）；模型组与GH组内膜厚度比较相对减小（P<0.01）；对照组和GH组之间的内膜厚度没有统计学差异。模型组与对照组内膜腺体、血管数量比较相对减少（P<0.01）；模型组与GH组内膜腺体、血管数量比较相对减少（P<0.01）；对照组与GH组内膜腺体、血管数量比较无统计学意义。模型组相对对照组波形蛋白、角蛋白表达分布少，且不平均，平均光密度值（MOD）减小（P<0.01）；模型组相对GH组的波形蛋白和角蛋白MOD值降低（P<0.01）；对照组相对GH组差异不明显。结论:宫腔灌注生长激素可以促进大鼠薄型子宫内膜再生和修复。

关键词: 生长激素, 宫腔灌注, 薄型子宫内膜, 动物模型

Abstract:

AIM: To study the therapeutic effect of intrauterine perfusion of growth hormone on thin endometrium. METHODS: Thirty female SD rats were randomly divided into three groups: control group, model group and GH group. The control group was not treated and routinely reared. The model group and GH group were perfused with 95% ethanol during estrus to establish a thin endometrial model. Six to eight hours after modeling, the model group was perfused with 0.2 mL normal saline, and the GH group was perfused with 0.2 mL growth hormone. HE staining was used to observe the thickness of endometrium, the number of blood vessels and glands. The expression of keratin and vimentin in rat endometrium was detected by immunoblotting and immunohistochemistry. RESULTS:The intima thickness, endometrial gland and intimal vessels in the GH group were increased compared with the model group (P<0.01). The intimal thickness, endometrial gland and intimal vessels were increased in the control group compared with the model group (P<0.01). There was no significant difference in intimal thickness, intimal glandular and intimal vessels between the control group and the GH group. The expression of vimentin and keratin in the GH group was increased (P<0.01). The expression of vimentin and keratin in the control group was increased (P<0.01). There was no significant difference in the expression of vimentin and keratin between the control group and the GH group. CONCLUSION:Intrauterine perfusion of growth hormone can promote the regeneration and repair of thin endometrium in rats.

Key words: growth hormone, intrauterine perfusion, thin endometrium, animal model

中图分类号:

R711

曹志文，胡卫华. 生长激素宫腔灌注治疗薄型子宫内膜的研究[J]. 中国临床药理学与治疗学, 2019, 24(9): 983-988.

CAO Zhiwen, HU Weihua. Effects of intrauterine perfusion of growth hormone on thin endometrium[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(9): 983-988.

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生长激素宫腔灌注治疗薄型子宫内膜的研究

Effects of intrauterine perfusion of growth hormone on thin endometrium

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