关键词: 芝麻素, 维生素E, 自发性高血压大鼠, 左心室纤维化, 转化生长因子β1, 结缔组织生长因子, 磷酸肌醇3激酶/蛋白激酶B

Abstract: AIM: Sesamin (Ses) is one of the major lignans in sesame seeds with antihypertensive and antifibroticactivities, but its effect is weak. In this experiment, we combined vitamin E (VitE) and Ses to observe their effects on blood pressure and left ventricular fibrosis in spontaneously hypertensive rats (SHRs), and to explore its possible mechanism.  METHODS: Male SHRs were randomly divided into SHRs+Ses+VitE group, SHRs+Ses group, SHRs+VitE group, and SHRs model group. Wistar-Kyoto (WKY) rats were setted as a normal control group. After 10 weeks of administration, systolic blood pressure (SBP) of rats was measured by tail cuff method, and the left ventricle was taken to calculate the organ index. Collagen deposition was observed by Masson staining, and cardiac collagen volume fraction (CVF) was analyzed. Expressions of collagen I, collagen III, transforming growth factor-β1 (TGF-β1), connective tissue growth factor (CTGF), matrix metalloproteinase-9 (MMP-9), matrix metalloproteinase inhibitor-1 (TIMP-1), phosphorylated protein kinase B (p-AKT), protein kinase B (AKT) and phosphoinositol 3 kinase (PI3K) were observed by Western blot. RESULTS: SBP, HW/BW, LVW/HW and CVF in SHRs+Ses+VitE group decreased significantly, and the decrease was higher than that in SHRs+Ses and SHRs+VitE group (P<0.01 or P<0.05). The expression of collagen I, collagen III, TGF-1, CTGF, MMP-9, TIMP-1, p-AKT and PI3K in SHRs+Ses+VitE group decreased significantly, and the decrease was higher than that in SHRs+Ses and SHRs+VitE group (P<0.01 or P<0.05). CONCLUSION: Ses combined with VitE can reduce the expression of TGF-1, CTGF, MMP-9 and TIMP-1 by inhibiting the activation of AKT/PI3K signaling pathway, thus exerting the effect of lowering blood pressure and improving left ventricular fibrosis.

Key words: sesamin, vitamin E, hypertension, fibrosis, TGF-β1, CTGF, PI3K/AKT