川陈皮素抑制高糖诱导的乳鼠心肌细胞肥大

doi:10.12092/j.issn.1009-2501.2021.07.005

中国临床药理学与治疗学 ›› 2021, Vol. 26 ›› Issue (7): 753-759.doi: 10.12092/j.issn.1009-2501.2021.07.005

川陈皮素抑制高糖诱导的乳鼠心肌细胞肥大

刘晓萍¹，赖香茂²，欧阳资章¹，江晟¹，张莹¹

¹广州医科大学附属第六医院清远市人民医院药学部，清远 511518，广东；²广州医科大学附属第六医院清远市人民医院泌尿外科，清远 511518，广东

收稿日期:2020-12-28 修回日期:2021-03-16 出版日期:2021-07-26 发布日期:2021-08-09
作者简介:刘晓萍，女，博士，副主任药师，研究方向：临床药学，心血管药理。 Tel: 0763-3113834 E-mail: 420411073@qq.com
基金资助:
广东省医学科研基金项目（A2021149）

Nobiletin inhibits neonatal rat cardiomyocytes hypertrophy induced by high glucose

LIU Xiaoping ¹, LAI Xiangmao ², OUYANG Zizhang ¹, JIANG Sheng ¹, ZHANG Ying ¹

¹Department of Pharmacy, the Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, Qingyuan 511518, Guangdong, China

Received:2020-12-28 Revised:2021-03-16 Online:2021-07-26 Published:2021-08-09

摘要/Abstract

摘要： 目的：探讨川陈皮素（nobiletin, Nob）抑制高糖诱导心肌细胞肥大的作用及其机制。方法：利用高糖（high glucose, HG）刺激乳鼠心肌细胞（neonatal rat cardiomyocytes, NRCMs）建立心肌细胞肥大模型，并给予Nob、核因子E2相关因子2（Nrf2）抑制剂Brusatol干预，采用MTT法检测细胞存活率，qRT-PCR法检测C-myc、Nppa mRNA水平，免疫荧光检测心肌细胞表面积，Western blot法检测Nrf2和血红素加氧酶-1（HO-1）蛋白表达水平。结果：33.3 mmol/L HG刺激NRCMs 48 h，细胞存活率显著下降，C-myc、Nppa mRNA水平和细胞表面积显著升高，Nrf2和HO-1蛋白表达显著降低（P<0.05）。给予Nob干预后，与HG组比较，细胞存活率、Nrf2和HO-1蛋白表达显著上调，C-myc、Nppa mRNA水平和细胞表面积显著下降（P<0.05）。利用Brusatol抑制Nrf2活性，与HG+Nob组比较，上述指标被逆转。结论：Nob抑制高糖诱导的心肌细胞肥大，其机制可能与激活Nrf2/HO-1信号通路有关。

关键词: 川陈皮素, 高糖, 心肌细胞肥大, Nrf2/HO-1信号通路

Abstract: AIM: To investigate the effect of nobiletin (Nob) on cardiomyocyte hypertrophy induced by high glucose and its mechanism. METHODS: Neonatal rat cardiomyocytes (NRCMS) were stimulated with high glucose (HG) to establish cardiomyocyte hypertrophy and nobiletin was given. Cell viability was measured by MTT assay. C-myc and Nppa mRNA levels were detected by qRT-PCR. Cellular surface area was detected by immunofluorescence, and Nrf2 and HO-1 protein expressions were detected by Western blot. RESULTS: After stimulation with 33.3 mmol/L HG for 48 h, the survival rate of NRCMS was significantly decreased, C-myc, Nppa mRNA levels and cellular surface area were significantly increased, Nrf2 and HO-1 protein expression were significantly decreased (P<0.05). After Nob treatment, compared with HG group, cellular surface area, Nrf2 and HO-1 protein expression were significantly increased, C-myc and Nppa mRNA levels were significantly decreased. The above indexes were reversed by using Nrf2 inhibitor. CONCLUSION: Nob inhibits cardiomyocyte hypertrophy induced by high glucose, and its mechanism may be related to the activation of Nrf2/HO-1 signaling pathway.

Key words: nobiletin, high glucose, cardiomyocyte hypertrophy, Nrf2/HO-1 signaling pathway

中图分类号:

R965.2

刘晓萍, 赖香茂, 欧阳资章, 江晟, 张莹. 川陈皮素抑制高糖诱导的乳鼠心肌细胞肥大[J]. 中国临床药理学与治疗学, 2021, 26(7): 753-759.

LIU Xiaoping, LAI Xiangmao, OUYANG Zizhang, JIANG Sheng, ZHANG Ying. Nobiletin inhibits neonatal rat cardiomyocytes hypertrophy induced by high glucose[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2021, 26(7): 753-759.

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川陈皮素抑制高糖诱导的乳鼠心肌细胞肥大

Nobiletin inhibits neonatal rat cardiomyocytes hypertrophy induced by high glucose

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