当归黄芪超滤物对电离辐射诱导下心肌细胞的影响及其机制研究

doi:10.12092/j.issn.1009-2501.2026.03.002

中国临床药理学与治疗学 ›› 2026, Vol. 31 ›› Issue (3): 300-312.doi: 10.12092/j.issn.1009-2501.2026.03.002

当归黄芪超滤物对电离辐射诱导下心肌细胞的影响及其机制研究

王夏颖¹(), 蒋虎刚¹^,², 刘佳坤¹, 马静¹, 刘凯¹^,², 李应东¹^,², 赵信科¹^,²^,*()

1. 甘肃中医药大学，兰州 730000，甘肃
2. 甘肃中医药大学附属医院，兰州 730000，甘肃

收稿日期:2025-05-06 修回日期:2025-06-26 出版日期:2026-03-26 发布日期:2026-04-03
通讯作者: 赵信科 E-mail:1686610858@qq.com;zxkd412@163.com
作者简介:王夏颖，女，在读硕士研究生，研究方向：中西医结合防治心血管疾病方向。E-mail：1686610858@qq.com
基金资助:
国家自然科学基金资助项目（82360926）；甘肃省教育厅：优秀研究生“创新之星”项目（2025CXZX-939.）；甘肃中医药大学研究生创新基金资助项目（2025CXCY-007）

Effect of Angelica astragalus ultrafiltration on cardiomyocytes induced by ionizing radiation and its mechanism

Xiaying WANG¹(), Hugang JIANG¹^,², Jiakun LIU¹, Jing MA¹, Kai LIU¹^,², Yingdong LI¹^,², Xinke ZHAO¹^,²^,*()

1. Gansu University of Chinese Medicine, Lanzhou 730000, Gansu, China
2. Affiliated Hospital of Gansu University of Chinese Medicine, Lanzhou 730000, Gansu, China

Received:2025-05-06 Revised:2025-06-26 Online:2026-03-26 Published:2026-04-03
Contact: Xinke ZHAO E-mail:1686610858@qq.com;zxkd412@163.com

摘要/Abstract

摘要：

目的: 探究电离辐射对H9C2心肌细胞的影响及当归黄芪超滤物（RAS-RH）的干预效应。方法: 应用X射线建立H9C2损伤模型，并选用RAS-RH对其进行干预。通过CCK-8法检测各组H9C2的活力情况，鬼笔环肽染色观察细胞的肌丝骨架变化，JC-1染色及流式细胞术检测细胞线粒体膜电位（ΔΨm）变化，Hoechst33324染色及流式细胞术检测细胞的凋亡情况，蛋白免疫印迹法（Western blot）实验检测Drp1和HSP70相关蛋白的表达水平。结果: （1）X射线可抑制H9C2的增殖（P<0.05），破坏细胞肌丝骨架、降低细胞活力、改变ΔΨm以及促进细胞凋亡（P<0.01，P<0.05）；（2）RAS-RH干预后H9C2活力升高（P<0.01）、细胞肌丝骨架恢复，且细胞的增殖能力、ΔΨm以及细胞凋亡（P<0.01、P<0.05）均得以改善；（3）RAS-RH可通过调节Drp1及HSP70蛋白的相对表达量（P<0.05）来改善H9C2生物学功能。结论: 电离辐射通过破坏细胞肌丝骨架、降低细胞活力、下调ΔΨm以及促进细胞凋亡进程来破坏H9C2的生物学功能，而RAS-RH可通过调节Drp1及HSP70蛋白的相对表达水平以修复电离辐射所带来的H9C2损伤。

关键词: 放射性心脏损伤, 当归黄芪超滤物, 电离辐射, 心肌细胞

Abstract:

AIM: To investigate the effects of ionizing radiation on H9C2 cardiomyocytes and the intervention efficacy of Radix Angelicae Sinensis-Radix Astragali ultrafiltrate (RAS-RH). METHODS: An H9C2 cardiomyocyte injury model was established using X-ray irradiation, followed by intervention with RAS-RH. Cell viability was assessed using the CCK-8 assay. Phalloidin staining was employed to observe changes in the cytoskeletal structure of cardiomyocytes. JC-1 staining combined with flow cytometry was used to measure mitochondrial membrane potential (ΔΨm). Hoechst 33324 staining and flow cytometry were applied to evaluate apoptosis. Western blot was performed to determine the expression levels of Drp1 and HSP70 proteins. RESULTS: (1) X-ray irradiation significantly inhibited the proliferation of H9C2 cells (P<0.05), disrupted cytoskeletal integrity, reduced cell viability, altered mitochondrial membrane potential (ΔΨm), and promoted apoptosis (P<0.01, P<0.05). (2) Intervention with RAS-RH (Radix Astragali and Radix Angelicae Sinensis ultrafiltrate) markedly enhanced H9C2 cell viability (P<0.01), restored cytoskeletal organization, and improved proliferative capacity, ΔΨm, and apoptosis resistance (P<0.01, P<0.05). (3) Mechanistically, RAS-RH ameliorated H9C2 cell function by modulating the relative protein expression levels of Drp1 (Dynamin-related protein 1) and HSP70 (Heat shock protein 70) (P<0.05). CONCLUSION: Ionizing radiation impairs the biological function of H9C2 cardiomyocytes by disrupting the cytoskeletal structure, reducing cell viability, downregulating ΔΨm, and promoting apoptosis. In contrast, RAS-RH can mitigate radiation-induced cardiomyocyte damage by regulating the relative expression levels of Drp1 and HSP70 proteins.

Key words: radiation-induced heart injury, radix angelicae sinensis and radix hedysari, ionizing radiation, cardiomyocytes

中图分类号:

R965.2

王夏颖, 蒋虎刚, 刘佳坤, 马静, 刘凯, 李应东, 赵信科. 当归黄芪超滤物对电离辐射诱导下心肌细胞的影响及其机制研究[J]. 中国临床药理学与治疗学, 2026, 31(3): 300-312.

Xiaying WANG, Hugang JIANG, Jiakun LIU, Jing MA, Kai LIU, Yingdong LI, Xinke ZHAO. Effect of Angelica astragalus ultrafiltration on cardiomyocytes induced by ionizing radiation and its mechanism[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2026, 31(3): 300-312.

图/表 14

图 1

Fig.1 Effect of ionizing radiation on viability of H9C2 cells by using CCK-8 method ($ \overline{x} $±s, n=3) bP<0.05, cP<0.01, compared with 0 Gy group.

图 2

Fig.2 The effects of ionizing radiation on myofibrillar skeleton of H9C2 cells were observed by guaninyl penicillamine staining (inverted microscope, 400×)

图 3

Fig.3 Effect of ionizing radiation on apoptosis of H9C2 cells with the Hoechst33342 staining (inverted microscope, 200×)

图 4

Fig.4 Effect of ionizing radiation on total apoptosis rate of H9C2 cells with the flow cytometry assay ($ \overline{x} $±s, n=3) bP<0.05, cP<0.01, compared with blank group.

图 5

Fig.5 Detection of the effect of ionizing radiation on mitochondrial transmembrane potential in H9C2 cells at 24 and 48 hours using JC-1 staining

图 6 cP<0.01, compared with blank group; eP<0.05, fP<0.01, compared with 2 Gy group.

Fig.6 Effect of ionizing radiation on mitochondrial transmembrane potential of H9C2 cells with the flow cytometry assay ($ \overline{x} $±s, n=3) cP<0.01, compared with blank group; eP<0.05, fP<0.01, compared with 2 Gy group.

图 7

Fig.7 Effect of RAS-RH on viability of ionizing radiation-induced H9C2 cells by using CCK-8 method ($ \overline{x} $±s, n=3) cP<0.01, compared with blank group; fP<0.01, compared with model group.

图 8

Fig.8 Effect of RAS-RH on muscle silk skeleton of ionizing radiation-induced H9C2 cells by using the ghost closed-loop peptide staining (inverted microscope, 400×)

图 9

Fig.9 Effect of RAS-RH on apoptosis of ionizing radiation-induced H9C2 cells by using Hoechst33342 staining (inverted microscope, 400×)

图 10

Fig.10 Effect of RAS-RH on ionizing radiation-induced apoptosis in H9C2 cells was determined by flow cytometry ($ \overline{x} $±s, n=3) cP<0.01, compared with blank group; fP<0.01, compared with model group.

图 11

Fig.11 Effect of RAS-RH on mitochondrial transmembrane potential of ionizing radiation-induced H9C2 cells by using JC-1 staining (inverted microscope, 400×)

图 12 bP<0.05, compared with blank group; eP<0.05, fP<0.01, compared with model group.

Fig.12 The effect of RAS-RH on the ionizing radiation-induced mitochondrial transmembrane potential in H9C2 cells was examined by JC-1 flow cytometry ($ \overline{x} $±s, n=3) bP<0.05, compared with blank group; eP<0.05, fP<0.01, compared with model group.

图 13 bP<0.05, cP<0.01, compared with 0 Gy group.

Fig.13 Effect of Drp1 and HSP70 proteins expression in ionizing radiation-induced H9C2 cells by using Western blot technology ($ \overline{x} $±s, n=3) bP<0.05, cP<0.01, compared with 0 Gy group.

图 14 cP<0.01, compared with blank group; eP<0.05, fP<0.01, compared with model group.

Fig.14 Effect of RAS-RH on the Drp 1 and HSP70 proteins expression in ionizing radiation-induced H9C2 cells by using Western blot technology ($ \overline{x} $±s, n=3) cP<0.01, compared with blank group; eP<0.05, fP<0.01, compared with model group.

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当归黄芪超滤物对电离辐射诱导下心肌细胞的影响及其机制研究

Effect of Angelica astragalus ultrafiltration on cardiomyocytes induced by ionizing radiation and its mechanism

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