云芝多糖对急性肺损伤小鼠的保护作用及对肠道菌群的影响

doi:10.12092/j.issn.1009-2501.2026.05.004

摘要/Abstract

摘要：

目的: 探究云芝多糖对急性肺损伤（ALI）小鼠的保护作用及对肠道菌群的影响。方法: 将24只小鼠随机分成三组，FC组（空白组）、FM组（LPS模型组）、FY组（云芝多糖预防组）。FY组的小鼠每天灌胃200 mg/kg云芝多糖，FC组和FM组的小鼠每天灌胃等量的生理盐水，连续灌胃处理15 d；24 h后对FM组和FY组的小鼠进行气管滴注13 mg/kg LPS，建立ALI模型。造模12 h后，对小鼠采样。检测小鼠血清中IL-6、IL-10、IL-β、TNF-α、T-AOC、SOD、GSH-Px和MDA的水平；采用HE染色观察肺组织病理形态学变化；采用测序技术分析小鼠肠道中菌群变化。结果: 与FM组比较，FY组小鼠肺组织的炎症细胞浸润均减少，肺泡隔增厚、肺泡水肿和毛细血管充血等情况均有所改善；血液中IL-6、IL-1β、TNF-α水平均显著降低，抗氧化能力显著提高。肠道菌群分析发现云芝多糖调节了小鼠肠道菌群的相对丰度，增加乳酸杆菌、放线菌、双歧杆菌等益生菌的相对丰度，并降低了普雷沃菌等有害菌的相对丰度，维持了肠道稳态。结论: 云芝多糖可以通过减轻炎症反应、增强抗氧化能力和调节小鼠肠道菌群来发挥抗ALI的作用。

关键词: 云芝多糖, 急性肺损伤, 小鼠, 肠道菌群

Abstract:

AIM: To investigate the protective effect of Coriolus versicolor polysaccharide on acute lung injury (ALI) mice and its influence on the intestinal microbiota. METHODS: Twenty-four mice were randomly divided into three groups: FC group (control group), FM group (LPS induced group), and FY group (Cordyceps polysaccharide prevention group). Mice in the FY group were given 200 mg/kg Coriolus versicolor polysaccharide by gavage daily, while mice in the FC group and FM group were given the same volume of normal saline by gavage daily for 15 days. 24 hours later, LPS (13 mg/kg) was intratracheally instilled into mice in the FM group and FY group to establish the ALI model. 12 hours after the model was established, samples were collected from the mice. The levels of IL-6, IL-10, IL-1β, TNF-α, T-AOC, SOD, GSH-Px, and MDA in the serum of the mice were detected; the pathological morphology changes of the lung tissue were observed via HE staining; and the changes in the intestinal microbiota of the mice were analyzed by sequencing technology. RESULTS: Compared with the FM group, the inflammatory cell infiltration in the lung tissue of mice in the FY group was reduced, and the thickening of the alveolar septum, pulmonary edema, and congestion of capillaries were improved; the levels of IL-6, IL-1β, and TNF-α in the blood were significantly decreased, and the antioxidant capacity was significantly increased. The analysis of the intestinal microbiota revealed that Coriolus versicolor polysaccharide regulated the relative abundance of the intestinal microbiota in mice, increasing the relative abundance of probiotics such as Lactobacillus, Actinomyces, and Bifidobacterium, and reducing the relative abundance of harmful bacteria such as Prevotella, maintaining intestinal homeostasis. CONCLUSION: Coriolus versicolor polysaccharide can exert anti-ALI effects by reducing inflammatory responses, enhancing antioxidant capacity, and regulating the intestinal microbiota of mice.

Key words: Coriolus versicolor polysaccharide, ALI, mice, intestinal microbiota

中图分类号:

R965.2

曹荣香, 李勇. 云芝多糖对急性肺损伤小鼠的保护作用及对肠道菌群的影响[J]. 中国临床药理学与治疗学, 2026, 31(5): 607-616.

Rongxiang CAO, Yong LI. Coriolus versicolor polysaccharide's protective effect on mice with acute lung injury and its influence on gut microbiota[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2026, 31(5): 607-616.

图/表 14

图 1

Fig.1 Tracheal instillation method and experimental process

表 1

Table 1 Body weight of mice in each group ($\bar x \pm s$, n=8)

Item	FC	FM	FY
Initial body weight (g)	23.03±0.59	23.03±0.51	23.04±0.65
Final body weight (g)	28.49±2.01	28.47±1.93	29.49±1.97
Total weight gain (g)	5.46±1.53	5.44±1.81	6.44±1.75^b
Average daily weight gain (g)	0.30±0.08	0.30±0.10	0.35±0.10
Relative growth rate (%)	23.60±6.04	23.63±7.72	27.95±7.59^cf

图 2

Fig.2 Morphological changes in lung tissue of mice in each group (HE staining, 200×)

图 3

Fig.3 Lung indices of mice in each group ($\bar x \pm s $, n=8) bP<0.05, cP<0.01, compared with FM group.

图 4

Fig.4 Inflammatory cytokine levels in each group of mice ($ \overline{x} $±s, n=8) bP<0.05, cP<0.01, compared with FM group.

图 5

Fig.5 Antioxidant indicators of mice in each group ($ \overline{x} $±s, n=8) cP<0.01, compared with FM group.

表 2

Table 2 Statistical table of sequencing quantity of mouse samples in each group

Sample	Input	Filtered	Denoised	Merged	Non-chimeric	Non-singleton
FC1	95974	79998	79005	74111	66433	66415
FC2	99486	84913	84203	81513	77855	77842
FC3	96753	83239	82633	80781	77835	77832
FM1	99012	85626	84701	80255	69971	69954
FM2	88376	75943	75005	70268	53127	53072
FM3	100046	87187	86413	82583	73698	73678
FY1	110707	95362	94197	89312	80651	80634
FY2	114102	98349	97584	94328	87144	87118
FY3	102873	88575	88044	86201	80742	80737

图 6

Fig.6 Venn diagram Each ellipse represents a sample (group), and the overlapping area between ellipses indicates the common ASV/OTU between samples (groups). The number of each block indicates the number of ASV/OTUs contained in that block.

图 7

Fig.7 Alpha Diversity Analysis A: Grouping box plot of Alpha diversity index; B: Species accumulation curve plot; C: Abundance level curve plot.

图 8

Fig.8 Beta diversity analysis

图 9

Fig.9 Bar chart of species composition of mice in each group at the phylum (A) and genus (B) levels

图 10

Fig.10 Microbial communities with significant differences at the phylum level among different groups of mice ($ \overline{x} $±s, n=8) bP<0.05, cP<0.01, compared with FC group.

图 11

Fig.11 Microbial communities with significant differences at the genus level among different groups of mice ($ \overline{x} $±s, n=8) bP<0.05, cP<0.01, compared with FC group; eP<0.05, fP<0.01, compared with FM group.

图 12

Fig.12 LEfSe analysis A: display of inter group difference classification units based on classification level tree Figure; B: LDA effect value bar chart of marker species.

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