关键词: 云南白药, 碘乙酸钠, 膝骨关节炎, 疼痛, 抗炎

Abstract:

AIM: To study the mechanism of Yunnan Baiyao on MIA-induced knee osteoarthritis pain of rats. METHODS: Sprague-Dawley male rats (160-220) g were randomly divided into 4 groups: Normal group (B), Control group (C) ,Yunnan Baiyao group (YB, 125 mg/kg) and Diclofenac sodium group (DF, 2.5 mg/kg). Then they were given single intra-articular injection with 2 mg MIA through the infrapatellar ligament of the right knee except for the normal group (B). The Paw withdrawal threshold (PWT) and cold pain latency of the right hind limb of each rats were collected as the baseline the day before making the animal model, followed by measuring the PWT and cold pain latency twice a week. The serum PGE2 level was determined by ELISA 35 days after administration, and the knee joint sections were observed via the microscope after H&E staining. Evaluation of drugs was estimated according to the Markin's score and the data was analyzed by SPSS18.0. RESULTS: Compared with the blank group, the mechanical pain threshold of the model group was significantly reduced (P<0.01), and the level of PGE2 in serum was increased (P<0.01). Compared with the model group, Yunnan Baiyao and diclofenac sodium can significantly increase the mechanical pain threshold of knee osteoarthritis in rats (P<0.01), reduce the serum PGE2 level of knee osteoarthritis in rats (P<0.05), and delay the cartilage injury of knee osteoarthritis.CONCLUSION:Yunnan Baiyao possibly relieves the pain of knee osteoarthritis induced by MIA through anti-inflammation.

Key words: Yunnan Baiyao, sodium iodoacetate, knee osteoarthritis, pain, anti-inflammation