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中国临床药理学与治疗学 ›› 2011, Vol. 16 ›› Issue (1): 5-12.

• 基础研究 • 上一篇    下一篇

三种茶饮料对小鼠肠道首过效应及肝脏Cyp3a、Cyp2e1的影响

林梅, 奇锦峰, 王永辉, 刘建敏, 韩坚   

  1. 广州中医药大学中药学院药理学教研室,广州 510006,广东
  • 收稿日期:2010-11-09 修回日期:2010-12-23 发布日期:2020-09-16
  • 通讯作者: 奇锦峰,男,博士,教授,硕士生导师,研究方向:药代动力学(药物相互作用)。Tel: 13711301907 E-mail: qijinfeng2005@yahoo.com.cn
  • 作者简介:林梅,女,硕士研究生,研究方向:药代动力学。Tel: 13726749815 E-mail: maylin2006lm@126.com

Effects of three kinds of tea beverages on intestinal first-pass effect and liver Cyp3a, Cyp2e1 activities in mice

LIN Mei, QI Jin-feng, WANG Yong-hui, LIU Jian-min, HAN Jian   

  1. Department of Pharmacology,College of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong,China
  • Received:2010-11-09 Revised:2010-12-23 Published:2020-09-16

摘要: 目的: 研究市售罐装绿茶、冰红茶及茉莉清茶饮料对小鼠肠道首过效应和肝脏细胞色素氧化酶(cytochrome P450,CYP)Cyp3a、Cyp2e1的影响。方法: SPF级小鼠随机分为6组,其中绿茶组、冰红茶组、茉莉清茶组小鼠分别自由饮用绿茶、冰红茶、茉莉清茶 7 d。用血中扑热息痛(acetaminophen,APAP)的浓度推断肠道首过效应。采用差速离心法分离肝/肠微粒体,以Bradford法定量蛋白,紫外分光光度法定量Cyp3a、Cyp2e1及血中APAP浓度。结果: 绿茶组血中APAP浓度较空白组明显升高(P<0.01),而冰红茶组与空白组相比则显著降低(P<0.01);绿茶组和冰红茶组小肠Cyp3a酶活性较空白组显著升高(P<0.05 或P<0.01)。各供试物组肝脏Cyp3a酶活性较空白组明显升高 (P<0.01),各供试物组肝脏Cyp2e1酶活性较空白组显著降低(P<0.01)。结论: 绿茶、冰红茶能显著改变肠道首过效应(绿茶抑制肠道P-gp,诱导肠道Cyp3a;冰红茶则诱导肠道P-gp及肠道Cyp3a;而茉莉清茶对肠道首过效应无影响)。各供试物均诱导肝脏Cyp3a的酶活性,抑制肝脏Cyp2e1的酶活性。故在服用经CYP3A、CYP2E1和P-gp代谢/转运的药物期间,大量或经常饮用上述茶可能影响这些药物的临床疗效和/或不良反应。

关键词: 茶饮料, Cyp3a, Cyp2e1, P-糖蛋白

Abstract: AIM: To investigate the effect of can packaged commercially available green tea, ice black tea and jasmine tea on intestinal first-pass effect and liver Cytochrome P450 (CYPs) Cyp3a, Cyp2e1 activities in mice.METHODS: SPF grade mice were randomly divided into 6 groups, green tea group, ice black tea group, jasmine tea group mice were getting freely access to green tea, ice black tea, jasmine tea respectively for 7 days. Intestinal first-pass effect was inferred through serum acetaminophen (APAP) levels. Liver/intestinal microsomes were prepared by differential centrifugation, and microsomal protein was quantified by Bradford method. Cyp3a, Cyp2e1 activities and serum APAP concentrations were assayed by spectrophotometry.RESULTS: APAP concentrations in green tea group were significantly higher than those in control group (P<0.01), while the levels were notably lower in ice black tea group than those in control group (P<0.01), the intestinal Cyp3a activities in green tea group and ice black tea group were considerably higher than those in control group (P<0.05 or P<0.01). Compared with control group, liver Cyp3a activities in all tea treated groups were greatly increased than those in control group (P<0.01). Compared with control group, liver Cyp2e1 activities in all tea treated groups were obviously reduced (P<0.01).CONCLUSION: Green tea and iced black tea seem significantly change the intestinal first-pass effect (green tea inhibit the activities of intestinal P-gp but induce intestinal Cyp3a, and ice black tea induce both intestinal P-gp and Cyp3a activities), while jasmine tea has no effect on intestinal first-pass effect. All three kinds of tea can significantly induce liver Cyp3a activities and inhibit liver Cyp2e1 activities. Therefore, intensely or frequently consuming these tea beverages during taking the medicines by which are metabolized or transported CYP3A, CYP2E1 and P-gp probably affect the drugs' clinical efficacy.

Key words: Tea beverages, Cyp3a, Cyp2e1, P-glycoprotein

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