胡桃醌对血管形成的影响及其机制初探

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (4): 403-409.

胡桃醌对血管形成的影响及其机制初探

陈丽¹, 张建², 汪思应¹, 黄德武¹, 顾为望²

¹安徽医科大学实验动物中心,合肥 230032,安徽;
²南方医科大学实验动物中心暨比较医学研究所,广州 510515,广东

收稿日期:2010-02-25 修回日期:2010-03-25 发布日期:2020-09-17
通讯作者: 顾为望,男,教授,研究方向:转基因动物制作、比较医学研究、实验动物新品种培育。Tel:021-61648043 E-mail:guww100@163.com
作者简介:陈丽,女,讲师,研究方向:比较医学。Tel: 0551-5167715 E-mail: yaligj@126.com

Effect of Juglone on the angiogenesis mmicrovessel structure of rat aorta and chick chorioallantoic membrane

CHEN Li¹, ZHANG Jian², WANG Si-ying¹, HUANG De-wu¹, GU Wei-wang²

¹Laboratory Animal Center,Anhui Medical University,Hefei 230032, Anhui, China;
²Laboratory Animal Center＆Department of Comparative Medicine, Southern Medical University, Guangzhou 510515,Guangdong,China

Received:2010-02-25 Revised:2010-03-25 Published:2020-09-17

摘要/Abstract

摘要： 目的: 观察胡桃醌对内皮细胞系EAHY.926细胞活性和迁移性、大鼠主动脉微血管样结构和鸡胚尿囊膜血管生成的影响。方法: 不同浓度胡桃醌作用于EAHY.926细胞株48 h后MTT法检测细胞存活率。大鼠主动脉无血清培养,加入100、50、25、12.5 μmol/L 浓度的胡桃醌,观察其对微血管结构的影响。选用7日龄鸡胚进行鸡胚绒毛尿囊膜血管生成实验。结果: 200、100、50 μmol/L 浓度胡桃醌对EAHY.926细胞活性有明显的抑制作用(P<0.05),IC₅₀为 122.848 μmol/L;25、12.5 μmol/L 的胡桃醌促进EAHY.926细胞株的生长(P<0.05)。100、50、25 μmol/L 的胡桃醌均可抑制大鼠主动脉微血管形成(P＜0.01),12.5 μmol/L 的胡桃醌可明显减少内皮细胞的爬行数量,延缓其爬行速度(P＜0.01)。200、100、50、25 μmol/L 胡桃醌对鸡胚绒毛尿囊膜已存在的血管有不同程度的刺激作用,出现不同程度的充血、出血,最终凝血,且呈剂量依赖性;而 12.5 μmol/L 的胡桃醌作用区域无新生血管形成。结论: 胡桃醌具有抗血管生成作用。

关键词: 胡桃醌, 抗血管生成, 主动脉环, EAHY.926, 鸡胚绒毛尿囊膜实验

Abstract: AIM: To investigate the effect of Juglone on the growth and migration of endothelial cell line EAHY.926, the microvessel structure of rat aorta and chick chorioallantoic membrane angiogenesis to provide pre-experimental data for the research of the mechanism of Juglo-ne in preventing tumor angiogenesis factor. METHODS: The effect of a series of concentration of Ju glone on the growth of EAHY.926 were measured by MTT. Serum-free culture of the rat aorta was conducted, with 100 μmol/L,50 μmol/L,25 μmol/L and 12.5 μmol/L dosages of Juglone added, to observe its effect on the microvessel structure. 7-day-old chick embryo were elected for experimental angiogenesis model. RESULTS: We found that 200 μmol/L, 100 μmol/L and 50 μmol/L dosages of Juglone could effectively inhibit the proliferation of EAHY.926 in a dose-dependent manner(P<0.05), with an IC₅₀ of 122.848 μmol/L according to the results of MTT. While 25 μmol/L,12.5 μmol/L and 6.25 μmol/L dosages of Juglone could promote growth of EAHY.926(P<0.05). 100 μmol/L,50 μmol/L and 25 μmol/L dosages of Juglone could completely inhibit the formation of new vessels,and 12.5 μmol/L dosages of Juglone could reduce the number of endothelial cells. 200 μmol/L,100 μmol/L,50 μmol/L and 25 μmol/L Juglone had vascular stimulation, hemolyzation, agglutination as a dose-dependent manner. 12.5 μmol/L Juglone could inhibit angiogenesis. CONCLUSION: Juglone can inhibit the formation of new vessels.

Key words: Juglone, Prevent vessel formation, Aorta, EAHY.926, Chick chorioallantoic membrane

中图分类号:

R965.2

陈丽, 张建, 汪思应, 黄德武, 顾为望. 胡桃醌对血管形成的影响及其机制初探[J]. 中国临床药理学与治疗学, 2010, 15(4): 403-409.

CHEN Li, ZHANG Jian, WANG Si-ying, HUANG De-wu, GU Wei-wang. Effect of Juglone on the angiogenesis mmicrovessel structure of rat aorta and chick chorioallantoic membrane[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(4): 403-409.

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