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中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (8): 895-899.

• 基础研究 • 上一篇    下一篇

丁酸钠诱导结肠癌HT-29细胞凋亡的信号通路及对AQP3基因表达影响的研究

朱静1, 洪华容2, 刘晓群1, 周晓莉1, 陈建丽1   

  1. 1四川省卫生学校, 成都610100, 四川;
    2成都医学院基础医学院, 成都610083, 四川
  • 收稿日期:2008-04-12 修回日期:2008-06-30 出版日期:2008-08-26 发布日期:2020-10-12
  • 作者简介:朱静, 女, 讲师, 从事药理学研究与教学。Tel:028-89291013 E-mail:zhujingsccd@yahoo.cn

Study on the signal pathway of cell apoptosis in HT-29 cells and the gene expression of AQP3 induced by sodium butyrate

ZHU Jing1, HONG Hua-rong2, LIU Xiao-qun1, ZHOU Xiao-li1, CHEN Jian-li   

  1. 1Sichuan Provincial School of Health, Sichuan 610100, Chengdu, China;
    2School of Basic Medical Sciences, Chengdu Medical College, Sichuan 610083, Chengdu, China
  • Received:2008-04-12 Revised:2008-06-30 Online:2008-08-26 Published:2020-10-12

摘要: 目的:探讨丁酸钠诱导结肠癌HT-29细胞凋亡过程的分子机理及其对水通道蛋白AQP3基因表达的影响。方法:MTT 法检测不同浓度的丁酸钠对结肠癌HT-29细胞生长的影响;RT-PCR 检测不同浓度的丁酸钠对细胞周期蛋白Cyclin D1、CDK4、p16和AQP3的基因表达水平, 同时使用流式细胞仪检测细胞周期。结果:加入浓度超过2.5 mmol/L 的丁酸钠后, 结肠癌HT-29细胞生长受到抑制,Cyclin D1、CDK4和AQP3的表达水平下降, p16的表达水平上调, 细胞阻滞于G1/S 期。结论:浓度超过2.5 mmol/L 的丁酸钠可以诱导结肠癌HT-29细胞凋亡, 使其细胞周期阻滞于G1/S期, 且这种诱导作用是剂量依赖型的, 其分子通路可能是通过p16-Cyclin D1/CDK4这条信号途径,在此过程中, 伴随着AQP3的基因表达水平降低。

关键词: 酸钠, HT-29, 细胞凋亡, G1/S 期阻滞, 信号通路, AQP3

Abstract: AIM:To investigate the signal pathway of cell apoptosis inHT-29 cells and the gene expression of AQP3 induced by sodium butyrate.METHODS: MTT method was used to detect the inhibitive effect of cell growth induced by different concentrations of sodium butyrate, while RT-PCR to detect the gene expressions of Cyclin D1, CDK4, p16 and AQP3, FCM for cell cycle detection.RESULTS:With the concentration of sodium butyrate more than 2.5 mmol/L, the cell growth of HT-29 cells was inhibited, the expressions of Cyclin D1, CDK4 and AQP3 were down-regulated while the expression of p16was up-regulated, cells were arrested in G1/S.CONCLUSION:The apoptosis of HT-29 cells may be induced by sodium butyrate with concentrations more than 2.5 mmol/L, while the cell cycle arrested in G1/S dose-dependently.The signal pathway of cell apoptosis may be the p16-Cyclin D1/CDK4 way, together with the down-regulation of AQP3.

Key words: sodium butyrate, HT-29, cell apoptosis, G1/S arrested, signal pathway, AQP3

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