靶控输注异丙酚不同麻醉深度对兔脑神经元型一氧化氮合酶mRNA水平的影响

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (6): 634-638.

靶控输注异丙酚不同麻醉深度对兔脑神经元型一氧化氮合酶mRNA水平的影响

陈建颜¹, 姚尚龙², 赵大忠¹, 卢健芳¹

¹深圳市第二人民医院麻醉科, 深圳 518035, 广东;
²华中科技大学同济医学院附属协和医院麻醉科, 武汉 430022, 湖北

收稿日期:2008-04-11 修回日期:2008-05-09 发布日期:2020-10-14
通讯作者: 陈建颜,男,博士,副主任医师,研究方向:异丙酚全麻作用机制。Tel:0755-83366388-8292 E-mail:medical90@sina.com

Changes of nNOS mRNA in rabbit brain under different anesthetic depths:using target-controlled infusion of propofol

CHEN Jian-yan¹, YAO Shang-long², ZHAO Da-zhong¹, LU Jian-fang¹

¹Department of Anesthesiology, Shenzhen Secondary People's Hospital, Shenzhen 518035, Guangdong, China;
²Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China

Received:2008-04-11 Revised:2008-05-09 Published:2020-10-14

摘要/Abstract

摘要： 目的: 研究异丙酚不同麻醉深度对兔脑神经元型一氧化氮合酶(nNOS)mRNA水平的影响。方法: 30只日本大耳兔,随机分为3组:对照组,浅麻醉组,深麻醉组,每组10只。浅麻醉组、深麻醉组予以异丙酚靶控输注,达到所需麻醉状态后1h断头处死。对照组行耳缘静脉注射空气造成空气栓塞后致死。动物处死后0～4℃分离取脑,原位杂交法测定小脑皮质、大脑顶叶皮质nNOSmRNA表达水平。结果: 与对照组比较,异丙酚麻醉状态下明显抑制兔大脑顶叶皮质、小脑皮质nNOSmRNA表达水平(P<0.05)。随麻醉深度的加深,小脑皮质nNOS表达水平进一步明显降低(P<0.05),但大脑顶叶皮质nNOS表达水平并未进一步降低(P>0.05)。结论: 异丙酚可能通过抑制nNOS活性,降低NO、环磷酸鸟苷(cGMP)水平,从而发挥麻醉作用。异丙酚对nNOS活性的抑制存在脑区差异。

关键词: 异丙酚, 靶控输注, 麻醉深度, 神经元型一氧化氮合酶, 脑

Abstract: AIM: To investigate alterations of nNOS mRNA in discrete brain regions of rabbits under propofol anesthesia.METHODS: Thirty rabbits were randomly allocated to three groups with ten each: control group, light anesthetic group, deep anesthetic group. Experimentation groups achieved needing drugged state by using target-controlled infusion of propofol, after one hour the rabbits were executed. Control group ear intravenous injection air caused aeroembolism death.Three groups rabbits were isolated to obtain brains under 0-4 ℃, the levels of nNOS mRNA in cerebellar cortex, parietal cortex were observed under different anesthetic depths.RESULTS: Comparing with control group, the levels of nNOS mRNA both in light anesthetic group and in deep anesthetic group decreased significantly in cerebellar cortex and parietal cortex (P<0.05) of rabbit brain.With the increasing of anesthetic depth, significant decreases in nNOS mRNA levels were observed in cerebellar cortex (P<0.05), but no significant difference was found in parietal cortex(P>0.05).CONCLUSION: Propofol probably exerts its anesthetic action through depressing the activity of nNOS, decreasing the levels of NO and cGMP.The depression differs in different brain regions.

Key words: propofol, target-controlled infusion, anesthetic depth, neuronal nitric oxide synthase, brain

中图分类号:

R965

陈建颜, 姚尚龙, 赵大忠, 卢健芳. 靶控输注异丙酚不同麻醉深度对兔脑神经元型一氧化氮合酶mRNA水平的影响[J]. 中国临床药理学与治疗学, 2008, 13(6): 634-638.

CHEN Jian-yan, YAO Shang-long, ZHAO Da-zhong, LU Jian-fang. Changes of nNOS mRNA in rabbit brain under different anesthetic depths:using target-controlled infusion of propofol[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(6): 634-638.

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