1-(3-氟苯基)-5-甲基-2-(1H)吡啶酮对肝星状细胞的影响

中国临床药理学与治疗学 ›› 2007, Vol. 12 ›› Issue (7): 782-785.

1-(3-氟苯基)-5-甲基-2-(1H)吡啶酮对肝星状细胞的影响

李萍¹, 陶立坚², 胡国龄³, 谭德明³

¹天津市传染病医院, 天津市肝病研究所, 天津 300192;
²中南大学湘雅医院肾病研究所, ³传染病研究所, 长沙 410008, 湖南

收稿日期:2007-03-29 修回日期:2007-05-18 出版日期:2007-07-26 发布日期:2020-10-27
作者简介:李萍,女,博士,副主任医师,研究方向:肝纤维化的治疗。Tel:13920265719 E-mail:Lao22@yahoo.com.cn

Effects of 1-(3-fluorophenyl)-5-methyl-2-(1H) pyridone on hepatic stellate cells

LI Ping¹, TAO Li-jian², HU Guo-ling³, TAN De-ming³

¹Tianjin Research Institute of Liver Diseases, Tianjin Infectious Diseases Specialty Hospital, Tianjin 300192, China;
²Institute of Nephritic Diseases, ³Institute of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 300192, Hunan, China

Received:2007-03-29 Revised:2007-05-18 Online:2007-07-26 Published:2020-10-27

摘要/Abstract

摘要： 目的: 探讨吡啶酮类化合物1-(3-氟苯基)-5-甲基-2-(1H) 吡啶酮(FMP) 抗肝纤维化的机制。方法: MTT 法观察FMP 对肝星状细胞(HSC) 增殖的影响, 免疫细胞化学观察FMP 对HSC Ⅰ、Ⅲ型胶原表达的影响。结果: FMP 200、300、400、500、600 μg/mL在24、48 h 可抑制HSC 增殖, 显著强于阳性对照组(P<0.05) 。免疫细胞化学显示FMP 在200 μg/mL可抑制HSC Ⅰ、Ⅲ型胶原蛋白表达。结论: FMP 治疗肝纤维化的原理可能与抑制HSC 增殖和HSC Ⅰ、Ⅲ型胶原蛋白表达有关。

关键词: 1-(3-氟苯基)-5-甲基-2-(1H) 吡啶酮, 肝星状细胞, 肝纤维化

Abstract: AIM: To explore the mechanism of 1-(3-fluorophenyl)-5-methyl-2-(1H) pyridone (FMP) against hepatic fibrosis. METHODS: The inhibitory effect of FMP on hepatic stellate cells(HSC) proliferation was observed by MTT, and the expression levels of collagen type I, III protein of HSC were detected by immunocytochemistry. RESULTS: HSC proliferation was decreased after incubation with FMP for 48 h at the dosage range from 200 μg/mL to 600 μg/mL compared with that in control group, and FMP could also significantly decrease the protein expression of collagen type I, III at the dosage of 200 μg/mL. CONCLUSION: FMP can inhibit the proliferation of HSC and decrease the expression of type Ⅰ, Ⅲ collagen, which are related to the mechanism of FMP treating hepatic fibrosis.

Key words: 1-(3-fluorophenyl)-5-methyl-2-(1H) pyridone, hepatic stellate cell, hepatic fibrosis

中图分类号:

R965.2

李萍, 陶立坚, 胡国龄, 谭德明. 1-(3-氟苯基)-5-甲基-2-(1H)吡啶酮对肝星状细胞的影响[J]. 中国临床药理学与治疗学, 2007, 12(7): 782-785.

LI Ping, TAO Li-jian, HU Guo-ling, TAN De-ming. Effects of 1-(3-fluorophenyl)-5-methyl-2-(1H) pyridone on hepatic stellate cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(7): 782-785.

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