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中国临床药理学与治疗学 ›› 2007, Vol. 12 ›› Issue (7): 778-781.

• 基础研究 • 上一篇    下一篇

蝮蛇毒抗高凝状态酶对小鼠肝癌抑制作用及P53、C-MYC表达的影响

张正明1, 芮景1, 柴智明2   

  1. 1皖南医学院弋矶山医院普外科, 2皖南医学院手术学教研室, 芜湖 241001, 安徽
  • 收稿日期:2006-03-27 修回日期:2007-07-10 出版日期:2007-07-26 发布日期:2020-10-27
  • 通讯作者: 芮景, 男, 教授, 硕士生导师, 主要研究方向:消化道肿瘤。Tel:0553-5739102 E-mail:ruijing1956@sina.com
  • 作者简介:张正明,男,硕士,主治医师,主要研究方向:消化道肿瘤。Tel:0553-5739310 E-mail:zzmzgp@163.com
  • 基金资助:
    安徽省教育厅自然科学基金资助项目(2004kj347)

Effect of anti-hypercoagulability state enzyme of agkistrodon venom on hepatocarcinoma and expresion of P53, C-MYC in mice

ZHANG Zheng-ming1, RUI Jing1, CHAI Zhi-ming2   

  1. 1Department of General Surgery of Yijishan Hospital, 2Department of Operation, Wannan Medical College, Wuhu 241001, Anhui, China
  • Received:2006-03-27 Revised:2007-07-10 Online:2007-07-26 Published:2020-10-27
  • Contact: 安徽省教育厅自然科学基金资助项目(2004kj347)

摘要: 目的: 建立实验性小鼠肝癌原位移植模型, 在此基础上研究蝮蛇毒抗高凝状态酶(AHCSE) 的抑癌作用并探讨其可能的作用机制。方法: 将小鼠肝癌H22 细胞种植于小鼠腋窝皮下, 传代后取癌组织块种植于小鼠肝脏, 进行不同剂量蛇毒制剂治疗的探索。将荷肝癌H22 小鼠分成4 组:模型对照组、AHCSE低、中、高剂量组(0.5、1.0、2.0 mg/kg), 给药10 d 后, 根据瘤重计算抑瘤率, 用免疫组化SP 法计算P53、C-MYC 阳性细胞数。结果: 0.5、1.0、2.0 mg/kg AHCSE 的肿瘤抑制率分别为35.57%、50.38%、53.89%, P53 阳性细胞数为64、51、45, Cmyc阳性细胞数为46、38、34 。1.0、2.0 mg/kg AHCSE 对小鼠移植性肝癌H22 有较明显的抑制作用。结论: AHCSE 对小鼠原位移植肝癌H22 的生长具有一定的抑制作用, 其机制可能与抑制突变型P53、CMYC蛋白的表达有关。

关键词: 蛇毒, 小鼠肝癌, 机制

Abstract: AIM: To research the inhibitory effect of antihypercoagulability state enzyme(AHCSE) of agkistrodon venom on the hepatocarcinoma in Balb cmice bearing hepatocarcinoma (H22) strain in situ and its possible mechanisms. METHODS: Hepatic cancer H22 model in mice was established on axillary fossa (Balb c) subcutaneouly, to obtain post-passage carcino-tissue and raise on mice livers.All animals were divided into 4 groups, 10 mice each group:control group, low-AHCSE group(0.5 mg/kg), middle-AHCSE group(1.0 mg/kg) and high-AHCSE group(2.0 mg/kg).Different dosages of AHCSE were injected via vena caudalis every three days for three times.To explore therapic efficacy of different dosages of AHCSE on the basis of percent inhibition of neoplastic weight and positive cells of P53 and C-MYC. RESULTS: The experiment of aniso-dosage of AHCSE indicates, percent inhibition of tumor(PIT) was 35.57% in low-AHCSE group (0.5 mg/kg), the PIT in the middle-dosage group(1.0 mg/kg) was 50.38% and in high-AHCSE group (2.0 mg/kg) PIT was 53.89%.The expression of P53 protein was 64, 51 and 45 and the expression of CMYC protein was 46, 38 and 34 in the three dosages groups.Inhibitory effect on tumor was significantly inmiddle-AHCSE and high-AHCSE. CONCLUSION: AHCSE has the significantly inhibitory effect on tumor in dose dependent.AHCSE may inhibit the tumor via down regulation the expression of P53, C-MYC protein.

Key words: agkistrodon venom, mice hepatic cancer(H22), mechanism

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