关键词: 肌肉乙酰胆碱受体, 卵母细胞, mRNA, 电压钳技术, 方法学研究

Abstract: AIM: To establish an appropriate model for the study of the effects of muscle relaxant drug on the acetylcholine receptor and lay a methodological foundation for the study of the pharmacology and toxicology of muscle relaxant drugs on the level of cells and protein.METHODS: Poly (A)+mRNA isolated from the rat muscle by Promega's new magnesphere technique were microinjected into defolliculation's Xenopus oocytes to express functional ion channels.Voltage clamp technique was used for record the current of the three group's voltage-dependent ion channels.The three-group properties of current were analyzed.RESULTS: There was no statistically difference among three groups in mean resting potential and compensative current during holding at-60 mV.The inner receptor interference could be obliterated by oocyte defolliculation and the typical current of N₂-Ach receptor could be recorded.CONCLUSION: The model set up by the test may lay a methodological foundation for the study of the pharmacology and toxicology of muscle relaxant drugs on the level of cell and protein.

Key words: muscle, ACh receptor, oocytes, mRNA, patch-clamp techniques, methodology