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中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (3): 348-351.

• 研究原著 • 上一篇    下一篇

粉尘螨脂质体制备和对致敏小鼠免疫功能的影响

岳文燕, 周书林, 刘文艳, 唐小牛, 王少圣, 陈文魁   

  1. 皖南医学院寄生虫教研室, 芜湖241001, 安徽
  • 收稿日期:2005-01-05 修回日期:2005-03-08 出版日期:2005-03-26 发布日期:2020-11-18
  • 通讯作者: 陈文魁, 男, 教授, 硕士生导师, 研究方向:变态反应与分子生物学。Tel:0553-3932252 E-mail:chenwenkui@163.com
  • 作者简介:岳文燕, 女, 硕士, 研究方向:变态反应与分子生物学。Tel:0553-3932252 E-mail:yuewenyan2004@163.com
  • 基金资助:
    安徽省教委自然科学基金资助项目(No2004kj337)

Preparation of liposomes encapsulating dermatophagoides farinae antigen and it's influence on immunity of mites sensitized mice

YUE Wen-yan, ZHOU Shun-lin, LIU Wen-yan, WANG Shao-sheng, CHEN wen-kui   

  1. Department of Parasitology, Wannan Medical College, Wuhu 241001, Anhui, China
  • Received:2005-01-05 Revised:2005-03-08 Online:2005-03-26 Published:2020-11-18

摘要: 目的:制备粉尘螨脂质体, 并探讨其对粉尘螨(抗原) 致敏小鼠免疫功能的影响, 以了解粉尘螨脂质体脱敏效果及机制。方法:用粉尘螨(抗原) 致敏BALB/c 小鼠, 皮下注射不同剂量粉尘螨脂质体,ELISA 法分别检测小鼠血清总IgG、总IgE、螨特异性IgG (sIgG)、螨特异性IgE (sIgE) 滴度及IL-4、IFN-γ水平。结果:致敏组小鼠血清总IgE、sIgE、总IgG、sIgG 滴度及IL-4 水平较正常组升高(P<0.01), IFN-γ水平显著下降(P<0.01)。皮下注射粉尘螨脂质体后, 小(1 μg°g-1 体重)、中(3 μg°g-1 体重)、大(9 μg°g-1体重) 剂量组小鼠血清IL-4 水平较致敏组下降, IFN-γ水平显著升高, 与正常组相近(P>.05)。总IgE、sIgE 均显著下降, 并以小剂量组下降最明显, 与正常组相近, 但中、大剂量组仍未下降至正常水平;小、中剂量组IgG 及sIgG 滴度与致敏组相比显著升高, 尤以小剂量组升高最明显, 而大剂量组与致敏组相比无明显差异, 但仍较正常组显著升高。结论:所制备的粉尘螨脂质体可以对粉尘螨致敏小鼠产生脱敏作用, 其中以小剂量组脱敏效果最好。

关键词: 粉尘螨, 脂质体, 特异性IgE, 特异性IgG, 总IgE, 总IgG

Abstract: AIM: To investigate the changes on immunological function in antigen-sensitized mice injected by dermatophagoides farinaeliposomes (DF) subcutaneously.METHODS: BALB/c mice were sensitized with DF antigen injected subcutaneously.After injected by DF liposomes with 1, 3, and 9 μg°g-1 dosage respectively, the total IgE, total IgG, DF specific IgE(sIgE), DF specific IgG (sIgG), IL-4, and IFN-γwere detected by ELISA.RESULTS: The total IgE, sIgE, total IgG, and sIgG in the serum of sensitized mice increased, the level of IFN-γreduced and IL-4 increased.After injected subcutaneously by DF liposomes, the total IgE, sIgE and IL- 4 in the serum of mice of all the treatment groups reduced, and IFN-γraised, and the total IgG, sIgG raised in the serum of mice administrated with small andmedium dosage, but the low dosage was more obvious.CONCLUSION: DF liposomes have some effects on the immunological function in mite-sensitized mice in the low dosage.

Key words: dermatophagoides farinae, liposome, specific IgE, specific IgG, total IgE, total IgG

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