应用超声心动图评价阿霉素所致兔扩张型心肌病模型的实验研究

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (1): 48-51.

应用超声心动图评价阿霉素所致兔扩张型心肌病模型的实验研究

费洪文, 王新房¹, 谢明星¹, 何亚乐

广东省人民医院, 广东省心血管病研究所心内科, 广州 510100, 广东;
¹华中科技大学同济医学院协和医院超声科, 武汉 430022, 湖北

收稿日期:2004-10-08 修回日期:2004-12-08 出版日期:2005-01-26 发布日期:2020-11-19

Evaluation of adriamycin-induced dilated cardiomyopathy model in rabbits by echocardiography

FEI Hong-wen, WANG Xin-fang¹, XIE Ming-xing¹, HE Ya-le

Department of Cardiology, Guangdong Provincial Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangzhou 510100, Guangdong, China;
¹Departement of Ultrasonography, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China

Received:2004-10-08 Revised:2004-12-08 Online:2005-01-26 Published:2020-11-19
Contact: FEI Hong-wen, male, medical doctor, major in echocardiography Tel:020-83827812-41204 E-mail:hongwen fei@hotmail.com

摘要/Abstract

摘要： 目的: 探讨阿霉素诱导兔心肌病模型的可行性, 并利用经胸超声心动图进行评估。方法: 20 只兔每周静脉注射阿霉素2 mg·kg^-1, 共计8 周(总剂量16 mg·kg^-1), 另8 只每周注射同等剂量的生理盐水作为对照组。基础状态(未注药前)、12 周时分别进行兔超声心动图检查, 分别测量左室舒张期末、收缩期末内径, 室间隔和左室后壁厚度, 以及射血分数和短轴缩短率。12 周时阿霉素组和对照组分别取2只进行心肌病理切片检查。结果: 阿霉素组与基础状态相比, 12 周时左室舒张期末内径显著扩大(10.5±1.3 和15.8±2.1 mm, P <0.05), 收缩期末内径显著扩大(8.7±1.0 和10.3±1.1 mm, P <0.05), 射血分数显著降低(63.4±8.4 和38.8±7.6, P <0.05), 短轴缩短率显著降低(P <0.01),室间隔和左室后壁厚度无显著性变化(37.6±7.6和21.2±6.7, P>0.05);对照组左室舒张期末、收缩期末内径、室间隔和左室后壁厚度、射血分数和短周缩短率均无显著性变化(P>0.05) 。阿霉素组病理结果显示了与人类阿霉素心肌毒性相似的改变,心肌细胞变性、线粒体和肌浆网水肿、细胞内空泡增多。结论: 静脉注射阿霉素可以诱导形成非缺血性心肌病模型, 结果可靠, 成功率高。经胸超声心动图可以用来监测评估模型的建立与效果。

关键词: 超声心动图, 兔, 阿霉素, 心肌病, 动物模型

Abstract: AIM: To determine the efficacy and reliability of adriamycin-induced cardiomyopathy model in rabbits and to evaluate it by transthoracic echocardiography.METHODS: Twenty rabbits were given 2 mg·kg^-1of adriamycin intravenously once a week for 8 weeks (total dose, 16 mg·kg^-1) to induce the cardiomyopathy model.Other eight rabbits received 2 ml·kg^-1of normal saline once aweek for 8 weeks as control.The left ventricular dimensions, wall thickness, fractional shortening and ejection fraction were measured by transthoracic echocardiography on the 1st day as baseline and in the 12th week after treatment.2 rabbits from each group were sacrificed for histological study in the 12th week.RESULTS: Compared with the baseline conditions, the value for the left ventricular diameter of the diastole (LVEDD) and that of the systole (LVESD) increased significantly, while the value for ejection fraction (EF) and the fractional shortening (FS) decreased significantly after treated with adriamycin (P <0.05).The values for interventricular septal thickness (IVST) and left ventricular posterior wall thickness (PWT) did not differ after treatment (P>0.05).In the control group, no significant changes were found in all parameters between the 1st day and the 12th week (P>0.05).The characteristics of adriamycin cardiotoxicity in human patients such as cardiomyocytes degeneration, mitochondrial and sarcoplasmic reticular edema, numerous intracellular vacuoles were found in the driamycin-treated group in histological study. CONCLUSION: Intravenous injection of adriamycin can be used to induce a reliable rabbit model of non-ischemic dilated cardiomyopathy with a high success rate.Transthoracic echocardiography can be applied in monitoring the animal model's formation.

Key words: echocardiography, rabbits, adriamycin, cardiomyopathy, animal models

中图分类号:

R965.2

费洪文, 王新房, 谢明星, 何亚乐. 应用超声心动图评价阿霉素所致兔扩张型心肌病模型的实验研究[J]. 中国临床药理学与治疗学, 2005, 10(1): 48-51.

FEI Hong-wen, WANG Xin-fang, XIE Ming-xing, HE Ya-le. Evaluation of adriamycin-induced dilated cardiomyopathy model in rabbits by echocardiography[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(1): 48-51.

[1]	彭娟, 李然宜, 范琳琳, 李晓宇, 吕迁洲, 邹云增. 心肌肥厚和心室重构的药物治疗进展[J]. 中国临床药理学与治疗学, 2022, 27(4): 382-389.
[2]	王文娟, 齐鸣瑞, 田利民. Nrf2在糖尿病心肌病中的作用及中药干预研究进展[J]. 中国临床药理学与治疗学, 2022, 27(10): 1163-1170.
[3]	王晓琳, 高攀, 邹云增. 线粒体氧化应激与糖尿病心肌病的关系[J]. 中国临床药理学与治疗学, 2021, 26(9): 1080-1085.
[4]	丁雪明, 陈天鹏, 徐强, 何欢, 何明. 川芎嗪通过14-3-3γ/Bcl-2减轻阿霉素引起的心肌毒性[J]. 中国临床药理学与治疗学, 2021, 26(4): 361-367.
[5]	张伟萍, 江建军, 陈晓锋, 许莎莎. 栀子苷通过抑制VPO1/ERK1/2信号通路减轻糖尿病心肌病大鼠的心肌细胞凋亡[J]. 中国临床药理学与治疗学, 2021, 26(2): 129-136.
[6]	金乐, 蒋多晨, 陈洪晓, 刘苏, 陈昭琳, 居靖. 槲皮素通过GAS5调控GSK-3β/β-catenin逆转乳腺癌阿霉素耐药[J]. 中国临床药理学与治疗学, 2021, 26(12): 1344-1351.
[7]	潘战和, 王馨, 苏安, 张鹏, 纪浩南, 王小梅. 基于Notch 1/NF-κB/STAT3通路探讨塞来昔布逆转NK/T细胞淋巴瘤细胞阿霉素耐药的作用机制[J]. 中国临床药理学与治疗学, 2020, 25(12): 1330-1336.
[8]	曹志文，胡卫华. 生长激素宫腔灌注治疗薄型子宫内膜的研究[J]. 中国临床药理学与治疗学, 2019, 24(9): 983-988.
[9]	范剑峰，方译，郑春华. 沙库巴曲缬沙坦钠治疗慢性心力衰竭患者的临床疗效分析[J]. 中国临床药理学与治疗学, 2019, 24(7): 810-814.
[10]	郑琼娜，武俏俏，马哲乐，张贤菲，干方敏，孔庆名. 低分子肝素钠对小鼠实验性肝纤维化干预作用的初步研究[J]. 中国临床药理学与治疗学, 2019, 24(5): 511-516.
[11]	张明清，刘玲娟，田杰. EGCG升高cTnIR193H限制型心肌病模型小鼠未突变cTnI的表达水平[J]. 中国临床药理学与治疗学, 2019, 24(4): 376-382.
[12]	刘雷，李轶枭，万波，曾青. miR-200c通过激活Wnt/β-连环蛋白信号通路诱导膀胱癌细胞阿霉素耐药[J]. 中国临床药理学与治疗学, 2018, 23(5): 510-516.
[13]	刘文贵，汪有锦，马坤，戴国梁，沈湘蕾，汪维，司海鹏. 多途径给予榄香烯对兔肝的组织学影响[J]. 中国临床药理学与治疗学, 2018, 23(3): 277-282.
[14]	刘建刚, 王晓波. 子宫内膜异位症模型大鼠背根神经节神经元中TRPV1、TRPA1表达及其意义[J]. 中国临床药理学与治疗学, 2017, 22(6): 617-621.
[15]	张翠，张根葆，王继胜，陆晓华，李曙. 芒果苷对糖尿病大鼠心肌的保护作用[J]. 中国临床药理学与治疗学, 2016, 21(8): 859-862.