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中国临床药理学与治疗学 ›› 2024, Vol. 29 ›› Issue (2): 139-145.doi: 10.12092/j.issn.1009-2501.2024.02.003

• 基础研究 • 上一篇    下一篇

丙酮酸钠对2型糖尿病大鼠体外贮存红细胞形态结构和功能的影响

白 玉1,姚 娜1,解小艺1,吴曼迪1,崔应辉1,池永斌2,郭建荣1   

  1. 1海军军医大学附属公利医院麻醉与围术期医学部,上海  200135;
    2上海市炎症与慢病管理人工智能重点实验室,上海  200135

  • 收稿日期:2023-08-31 修回日期:2023-09-18 出版日期:2024-02-26 发布日期:2024-02-02
  • 通讯作者: 郭建荣,男,博士,博士后,主任医师,教授,博士生导师,研究方向:麻醉与血液保护。 E-mail:jianrguo@126.com
  • 作者简介:白玉,女,硕士研究生,研究方向:麻醉与血液保护。 E-mail: 1367849709@qq.com
  • 基金资助:
    上海市浦东新区卫生系统新兴交叉学科建设项目(PWXx2020-05);上海市浦东新区卫生系统重点学科群建设项目(PWZxq2022-05)

Effects of sodium pyruvate on the morphology, structure and function of erythrocytes stored in vitro in type 2 diabetes rats

BAI Yu1, YAO Na1, XIE Xiaoyi1, WU Mandi1, CUI Yinghui1, CHI Yongbin2, GUO Jianrong1   

  1. 1Department of Anesthesiology and Perioperative Medicine, Shanghai Gongli Hospital, Naval Military Medical University, Shanghai 200135, China; 2Shanghai Health Commission Key Lab of Artificial Intelligence (AI)-Based Management of Inflammation and Chronic Diseases, Shanghai 200135, China
  • Received:2023-08-31 Revised:2023-09-18 Online:2024-02-26 Published:2024-02-02

摘要:

目的:观察加有丙酮酸钠的红细胞保存液对2型糖尿病(T2DM)大鼠体外贮存红细胞(RBC)形态结构和功能的影响。方法:雄性SPF级SD大鼠30只,随机分为3组(n=10):非T2DM常规RBC保存液(A组)、T2DM常规RBC保存液(B组)、T2DM丙酮酸钠RBC保存液(C组)。尾静脉取血滤白细胞后,分别于贮存0 d(T0)、7 d(T1)、14 d(T2)、21 d(T3)、28 d(T4)观测各组RBC的形态结构,检测RBC中二磷酸甘油酸(2,3-DPG)、活性氧(ROS)、丙二醛(MDA)及乳酸(LA)的含量,对体外贮存14 d的RBC进行PKH26标记,于静脉回输后1 h、4 h、10 h、16 h测定RBC在体内的存活率。结果:T0时A组RBC形态完好,明显优于B组和C组;随贮存时间延长,各组RBC形态逐渐变成棘球形,B组和C组RBC棘形发生率明显高于A组,C组棘形发生率低于B组(P<0.05);与A组比较,不同时点B组和C组2,3-DPG含量下降,ROS、MDA增加(P<0.05),其中C组2,3-DPG含量高于B组、ROS和MDA含量低于B组(P<0.05);各时点B组LA含量均高于A组和C组(P<0.05),在T2-T4时C组LA含量低于A组(P<0.05);RBC标记回输后,A组RBC存活率明显高于B、C组,B组RBC存活率显著低于C组(P<0.05)。结论:添加丙酮酸钠的改良RBC保存液对2型糖尿病大鼠体外贮存RBC具有一定保护作用,其机制可能与抗氧化作用有关。

关键词: 丙酮酸钠, 2型糖尿病, 红细胞, 形态结构, 功能

Abstract:

AIM: To observe the effect of RBC preservation solution with sodium pyruvate on the morphology, structure and function of RBC stored in vitro in type 2 diabetes rats.?METHODS: Thirty SPF male SD rats,were randomly divided into 3 groups (n=10): non-T2DM conventional RBC preservation solution (group A), T2DM conventional RBC preservation solution (group B) and T2DM sodium pyruvate RBC preservation solution (group C).?The leukoreduced RBC from the tail vein and stored for 0 d (T0), 7 d (T1), 14 d (T2), 21 d (T3) and 28 d (T4) to detect the morphology, structure and the contents of 2,3-DPG, reactive oxygen species (ROS), malondialdehyde (MDA) and lactic acid (LA) of RBC in group A, B and C. The RBC stored for 14 days in vitro were labeled with PKH26, and its survival rate were tested in vivo at 1, 4, 10 and 16 hours after intravenous infusion. RESULTS: At T0, the RBC morphology of group A was intact, which was better than that of group B and group C. With the extension of storage time, the morphology of RBC in each group gradually transformed into a spindle-spherical shape. Compared with group A, the incidence of acanthocytes in group B and group C was higher, and the incidence of acanthocytes in group C was lower than that in group B. Compared with group A, the content of 2,3-DPG in group B and group C decreased, while ROS and MDA increased at different time points (P<0.05). The content of 
2,3-DPG in group C was higher than that in group B (P<0.05), and the contents of ROS and MDA were lower than those in group B (P<0.05). LA content in group B was higher than that in group A and group C (P<0.05). At T2-T4, the LA content in group C was lower than that in group A (P<0.05). The survival rate of RBC in group A was higher than that in group B and C, and the survival rate of RBC in group B was lower than that in group C (P<0.05). CONCLUSION: Sodium pyruvate added RBC preservation solution has a certain protective effect on RBC stored in vitro in type 2 diabetic rats, and its mechanism may be related to its antioxidant effect.

Key words: sodium pyruvate, type 2 diabetes, red blood cell, morphological structure, function

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