氯胺酮对神经病理性痛大鼠海马CA1区突触长时程增强的影响

中国临床药理学与治疗学 ›› 2011, Vol. 16 ›› Issue (2): 170-173.

氯胺酮对神经病理性痛大鼠海马CA1区突触长时程增强的影响

魏辉明, 麻伟青, 杨云丽, 董发团, 张承华, 王慧明

成都军区昆明总医院麻醉科,昆明 650032,云南

收稿日期:2010-12-13 修回日期:2011-01-12 发布日期:2011-04-20
作者简介:魏辉明,男,博士,副主任医师,研究方向:麻醉及疼痛临床药理学。Tel: 0871-4774724 E-mail: medicana@sina.com

Effect of ketamine on synaptic long-term potentiation in hippocampus in rats with neuropathic pain

WEI Hui-ming, MA Wei-qing, YANG Yun-li, DONG Fa-tuan, ZHANG Chen-hua, WANG Hui-ming

Department of Anesthesiology, Kunming General Hospital of Chengdu Military Area, Kunming 650032, Yunnan,China

Received:2010-12-13 Revised:2011-01-12 Published:2011-04-20

摘要/Abstract

摘要： 目的:探讨氯胺酮对神经病理性痛大鼠海马CA1区突触长时程增强(LTP)的影响。方法: 成年雄性Wistar大鼠18只,体重190~220 g,随机分为3组(n=6):神经病理性痛模型组、假手术组、氯胺酮组。采用结扎L_4,5左侧脊神经的方法制备大鼠神经病理性痛模型。于模型制备后1周、2周和3周观察大鼠痛行为学及足部形态;于模型制备前、后1周、2周和3周时测定痛阈;于最后一次痛阈测定结束后 3 d 记录海马CA1区兴奋性突触后电位(EPSP),以高频刺激(HFS)诱发LTP,氯胺酮组HFS前 20 min 经腹腔注射 25 mg/kg。结果: 与假手术组比较,模型组和氯胺酮组各时点痛阈降低;与模型组相比,氯胺酮组LTP程度减弱(P<0.01)。结论:氯胺酮可抑制神经病理性痛对大鼠海马CA1区突触LTP的易化作用。

关键词: 神经痛, 长时程增强, 海马, 氯胺酮

Abstract: AIM: To investigate the effect of ketamine on synaptic long-term potentiation (LTP) in the hippocampus in rats with neuropathic pain.METHODS: Eighteen adult male Wistar rats weighing 190-220 g were randomly divided into three groups (n=6): model of neuropathic pain group (model group),sham operating group, and ketamine group.Neuropathic pain was produced by ligation of the left L_4,5 spinal nerve.The behaviour of the rats was evaluated at 1, 2 and 3 week after surgery.The pain threshold was measured before surgery,at 1, 2 and 3 week after surgery.The excitatory post-synaptic potential (EPSP) in hippocampal CA1 region was measured at 3 d after the last measurement of pain threshold.LTP was induced by high-frequency stimulation(HFS).Ketamine(25 mg/kg i.p.)was injected at 20 min before HFS.RESULTS:Compared with the sham operating group,the pain threshold was significantly lower in the model group and ketamine group.Compared with the model group,the amplitude of LTP was significantly lower in the ketamine group (P<0.01).CONCLUSION: Ketamine might inhibit the facilitation of synaptic LTP induced by neuropathic pain in the CA1 area of the hippocampus.

Key words: Neuralgia, Long-term potentiation, Hippocampus, Ketamine

中图分类号:

R441.1

魏辉明, 麻伟青, 杨云丽, 董发团, 张承华, 王慧明. 氯胺酮对神经病理性痛大鼠海马CA1区突触长时程增强的影响[J]. 中国临床药理学与治疗学, 2011, 16(2): 170-173.

WEI Hui-ming, MA Wei-qing, YANG Yun-li, DONG Fa-tuan, ZHANG Chen-hua, WANG Hui-ming. Effect of ketamine on synaptic long-term potentiation in hippocampus in rats with neuropathic pain[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(2): 170-173.

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