肺癌复发转移患者外周血循环DNA的定量分析

中国临床药理学与治疗学 ›› 2011, Vol. 16 ›› Issue (2): 209-213.

肺癌复发转移患者外周血循环DNA的定量分析

刘宏杰, 叶斌, 程静, 吉兆宁

皖南医学院附属弋矶山医院肿瘤防治中心, 芜湖 241001, 安徽

收稿日期:2010-12-14 修回日期:2011-01-12 发布日期:2011-04-20
通讯作者: 吉兆宁,男,博士,教授,主任医师,硕士生导师,研究方向:恶性肿瘤的化学治疗和生物治疗。Tel: 0553-5739060 E-mail: jzning@163.com
作者简介:刘宏杰,男,在读硕士,研究方向:恶性肿瘤的化学治疗和生物治疗。Tel: 13955399570 E-mail: lhj15956397139@sina.com

Quantitative analysis of circulating DNA in plasma of lung cancer patients with metastasis or recurrence

LIU Hong-jie, YE Bin, CHENG Jing, JI Zhao-ning

Cancer Center,Yijishan Hospital of Wannan Medical College,Wuhu 241001, Anhui, China

Received:2010-12-14 Revised:2011-01-12 Published:2011-04-20

摘要/Abstract

摘要： 目的:检测肺癌患者外周血循环DNA的含量,探讨其与肿瘤复发转移的关系。方法: 收集98例肺癌患者、44例良性疾病患者和60例健康志愿者的外周血样本,抽提外周血循环DNA,以SYBR green I荧光染色法行DNA定量。结果: 肺癌患者外周血循环DNA浓度显著高于良性病变患者和健康自愿者,肺癌发生复发转移时外周血循环DNA的含量明显增高。结论:外周血循环DNA定量有可能成为一种新的与肺癌转移复发相关的恶性肿瘤标志物。

关键词: 肺癌, 外周血循环DNA, 转移, 复发

Abstract: AIM: To quantify the circulating DNA in plasma from patients with lung cancer and investigate the relationship with the circulating DNA and neoplasm metastasis or recurrence in lung cancer patients.METHODS: Blood samples were collected from 98 lung cancer patients,44 patients with non-neoplastic diseases,and 60 healthy volunteers. The circulating DNA was extracted and analyzed by fluorescent dye(SYBR green I)staining.RESULTS:The circulating DNA concentration in the lung cancer, was significantly higher than which patients with non-neoplastic diseases and the healthy volunteers.The level of circulating DNA was higher in lung caner patients with metastasis or recurrence.CONCLUSION: Circulating DNA in plasma may become a new tumor marker to monitor metastasis or recurrence of lung cancer.

Key words: Lung cancer, Circulating DNA, Metastasis, Recurrence

中图分类号:

R734

刘宏杰, 叶斌, 程静, 吉兆宁. 肺癌复发转移患者外周血循环DNA的定量分析[J]. 中国临床药理学与治疗学, 2011, 16(2): 209-213.

LIU Hong-jie, YE Bin, CHENG Jing, JI Zhao-ning. Quantitative analysis of circulating DNA in plasma of lung cancer patients with metastasis or recurrence[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(2): 209-213.

参考文献

[1] 胡影,倪虹.人体游离循环DNA的研究进展[J].遗传学报,2008,30(7):815-820.
[2] Wong BC, Lo YM.Cell-free DNA and RNA in plasma as new tools for molecular diagnostics[J].Expert Rev Mol Diagn,2003,3(6):785-797.
[3] Ziegler A,Zangemeister-Wittke U,Stahel RA, et al. Circulating DNA:a new diagnostic gold mine [J]?Caneer Treat Rev,2002,28(5):255-271.
[4] Jahr S,Hentze H,Englisch S,et a1.DNA fragments in the blood plasma of cancer patients:quantitations and evidence for their origin from apoptotic and necrotic cells[J].Cancer Res,2001,61(4):1659-1665.
[5] Leon SA,Shapiro B,Sklaroff DM,et a1.Free DNA in the serum of cancer patients and the effect of the therapy[J].Cancer Res,1977,37(3):646-650.
[6] Maebo A.Plasma DNA level as a tumor marker in primary lung cancer [J].Nihon Kyobu Shikkan Gakkai Zasshi,1990,28(8):1085-1091.
[7] Fournie GJ,Courtin JP,Laval F,et al.Plasma DNA as a marker of cancerous cell death.investigations in patients suffering from lung cancer and in nude mice bearing human tumours[J].Cancer lett,1995,91(2):221-227.
[8] Gautsehi O,Bigoseh C,Huegli B,et al.Circulating deoxyribonucleic acid as prognostic marker in non-small-cell lung cancer patients undergoing chemotherapy[J]. Clin Oncol,2004,22(20):4157-4164
[9] Sozzi G,Conte D,Mariani L,et a1.Analysis of circulating tumor DNA in plasma at diagnosis and during follow-up of lung cancer patients[J].Cancer Res, 2001,61(12):4675-4678.
[10]Pathak AK,Bhutani M,Kumar S,et a1.Circulating cell-free DNA inplasma／serum of lung cancer patients as a potential screening and prognostic tool[J].Clin Chem,2006,52(10):1833-1842.
[11]屠红,高海峰,傅士龙,等.肿瘤患者循环DNA的定量研究[J].中华肿瘤杂志,2004,26(10):606-608.
[12]今毅,谢文,余小萍,等. 乳腺癌患者肿瘤循环DNA的定量分析[J].第四军医大学学报, 2007,28(11):1024-1026.
[13]Silva JM,Silva J,Sanchez A,et al.Tumor DNA in plasma at diagnosis of breast cancer patients is a valuable predictor of diseases survival[J].Clin Cancer Res,2002,8(12):376l-3766.
[14]Kurusu Y,Yamashita J,Ogawa M,et al. Detection of circulating tumor cells by reverse transcriptase polymerase chain reaction in patients with resectable non-small-cell lung cancer[J]. Surgery, 1999,126(5):820-826.
[15]Tong YK,Lo YM.Diagnostic developments involving cell-free(circulating) nucleic acids[J].Clin Chim Acta,2006,363(1/2):187-196.
[16]Johnson P,Lo YMD.Plasma nucleic acids in the diagnosis and management of malignant disease[J].Clin Chem,2002,48(8):l l86-1193.
[17]吴仲南,周向军,王永祥,等.DNA甲基化与肿瘤诊断[J]. 中国临床药理学与治疗学,2006,11(2):131-135.
[18]Chen XQ, Stroun M,Magnenat JL,et al.Nature Med,1996,2(9):1033-1035.
[19]邓立春,许晨,姜藻,等.恶性肿瘤患者循环血DNA的研究进展[J].东南大学学报,2007,26(5):382-385.