大鼠创伤性脊髓损伤模型的建立

中国临床药理学与治疗学 ›› 2011, Vol. 16 ›› Issue (7): 721-728.

• 基础研究 • 下一篇

大鼠创伤性脊髓损伤模型的建立

顾兵¹, 金建波², 李华南², 王烁宇²

¹江西科技师范学院药学院,南昌 330013,江西;
²江西中医学院药学院,南昌 330006,江西

收稿日期:2011-05-30 修回日期:2011-06-11 出版日期:2011-07-26 发布日期:2011-09-22
作者简介:顾兵,男,博士后,副教授,硕士生导师,从事神经精神药物学研究。E-mail: bguemory@hotmail.com
基金资助:
国家自然科学基金资助项目(30960448);江西省教育厅科技项目(GJJ09546, GJJ11596)

Establishment of traumatic spinal cord injury model in rats

GU Bing¹, JIN Jian-bo², LI Hua-nan², WANG Shuo-yu²

¹School of Pharmacy, Jiangxi Science & Technology Normal University, Nanchang 330013, Jiangxi ,China;
²School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, Jiangxi ,China

Received:2011-05-30 Revised:2011-06-11 Online:2011-07-26 Published:2011-09-22

摘要/Abstract

摘要： 目的: 建立一种控制性脊髓挫伤大鼠模型。方法: 采用Benchmark^TM立体定位颅脑撞击器制备大鼠创伤性脊髓损伤,并在伤后1、2、3、5、7、14、28 d 借助斜板试验及BBB评分评价其运动功能,然后观察损伤部位及其邻近区域的形态学改变。结果: 脊髓损伤后大鼠在斜板上维持的角度以及BBB评分显著低于假手术组(P<0.01)。损伤部位可见神经纤维肿胀。灰质部运动神经元肿胀、坏死,尼氏体淡染,甚至溶解。小胶质细胞明显增生。通过铜银染色和Luxol固蓝染色,可见运动神经元溃变以及髓鞘脱失。结论: 建立的控制性脊髓挫伤大鼠模型重复性好,适合神经保护药物的药效学筛选。

关键词: 创伤性脊髓损伤, 动物模型, 大鼠, 挫伤, 铜银染色, Luxol固蓝染色

Abstract: AIM: To establish a rat model for controlled spinal cord contusion injury.METHODS: Traumatic spinal cord injury model (TSCI) in rats was duplicated by controlled spinal cord contusion injury method with a BenchmarkTM stereotaxic cortical impactor. Motor function was evaluated at 1, 3, 5, 7, 14, 28 day after TSCI with inclined plane test and BBB score. And then morphological changes were carefully inspected in the injured part and adjacent area.RESULTS: The angle sustaining on the inclined plane and BBB score of spinal cord injured rats were significantly lower than that of sham-operated rats(P<0.01). Nerve fibers were swollen in the injured sites. Motoneurons were swollen or loss and Nissl bodies were lightly stained or even dissolved in gray matter. Microglia remarkably proliferated. Motoneuron degeneration and demyelination were observed by using cupric-silver straining and Luxol Fast Blue staining technique.CONCLUSION: The rat model for controlled spinal cord contusion injury is reproducible and suitable for the pharmacodynamic screening of neuroprotective drugs.

Key words: Traumatic spinal cord injury, Animal model, Rat, Contusion, Cupric-silver staining, Luxol Fast Blue staining

中图分类号:

顾兵, 金建波, 李华南, 王烁宇. 大鼠创伤性脊髓损伤模型的建立[J]. 中国临床药理学与治疗学, 2011, 16(7): 721-728.

GU Bing, JIN Jian-bo, LI Hua-nan, WANG Shuo-yu. Establishment of traumatic spinal cord injury model in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(7): 721-728.

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大鼠创伤性脊髓损伤模型的建立

Establishment of traumatic spinal cord injury model in rats

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