水飞蓟宾对D-半乳糖诱导衰老大鼠抑制糖氧化应激反应及改善学习记忆作用

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (1): 10-14.

水飞蓟宾对D-半乳糖诱导衰老大鼠抑制糖氧化应激反应及改善学习记忆作用

傅永锦¹, 潘竞锵², 张小牧¹, 邝少松³

¹广州市中医医院,广州 510130,广东;
²广州市中医中药研究所,广州 510130,广东;
³广东省医学实验动物中心,佛山 528248,广东

收稿日期:2011-10-10 修回日期:2011-11-19 出版日期:2012-01-26 发布日期:2012-02-16
通讯作者: 潘竞锵,男,主任药师,从事抗炎-免疫、老年药学和生化药理学研究。E-mail: pjq56@yahoo.com.cn
作者简介:傅永锦,男,主管药师,从事药学以及实验药理学研究。Tel: 020-81226274 E-mail: fuyongjin@21cn.com

Effects of silybin on inhibiting glycoxidative stress and improving the learning and memory in aging rats treated by D-galactose

FU Yong-jin¹, PAN Jing-qiang², ZHANG Xiao-mu¹, KUANG Shao-shong³

¹Guangzhou Hospital of Traditional Chinese Medicine, Guangzhou 510130, Guangdong, China;
²Guangzhou Institute of Traditional Chinese Medicine & Materia Medica, Guangzhou 510130, Guangdong, China;
³Guangdong Medical Experimental Animal Center, Foshan 528248, Guangdong, China

Received:2011-10-10 Revised:2011-11-19 Online:2012-01-26 Published:2012-02-16

摘要/Abstract

摘要： 目的: 观察水飞蓟宾对D-半乳糖(D-gal)诱导衰老大鼠糖化氧化应激反应及学习记忆作用的影响。方法: 采用D-gal诱导衰老大鼠模型;采用Morris水迷宫测定法检测大鼠学习记忆能力。实验结束后测定大鼠血浆糖化血红蛋白(HbA1c)、血浆非酶糖基化终产物(AGEs)和果糖胺(FRA)含量、红细胞醛糖还原酶(AR)活性及脑组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)、AGEs含量。结果: 水飞蓟宾 150 mg/kg和75 mg/kg均能明显抑制D-gal诱导的AR活性增高(P<0.01),降低FRA、HbA1c和AGEs含量(P<0.01 或P<0.05),能不同程度地降低大鼠脑组织AGEs和MDA含量,提高SOD活性(P<0.01)。结论: 水飞蓟宾对D-半乳糖所致衰老大鼠学习记忆障碍具有保护作用,能增强学习记忆能力,其机制可能与抑制糖氧化应激反应有关。

关键词: 水飞蓟宾, 衰老, D-半乳糖, 糖氧化应激反应, 学习记忆

Abstract: AIM: To investigate the effects of silybin on the glycoxidative stress and the abilities of learning and memory in aging rats induced by D-galactose.METHODS: The aging rats induced by D-galactose, the abilities of learning and memory were measured by Morris water maze test in rats.To assess the glycoxidative stress, the glycosylated hemoglobin(HbA1c) and fructosamine (FRA), advanced glycation end products(AGEs) in plasma,the activity of aldose reductase(AR) in red blood cell (RBC) were used , the levels of malondialdehyde (MDA) and AGEs and ability of superoxide dismutase(SOD) in brain tissue were also acquired.RESULTS: Compared with the control group, Sily groups(150 mg/kg and 75 mg/kg ) obviously inhibited the activity of AR(P<0.01), reduced the levels of FRA, HbA1c and AGEs in rats treated by D-galactose(P<0.01 or P<0.05), and the levels of MDA and AGEs were reduced while the activity of SOD in brain were elevated(P<0.01).CONCLUSION: Silybin could improve the abilities of learning and memory in rats treated by D-galactose, and it might be related to the inhibition of glycoxidative stress and ability of AR.

Key words: Silymarin, Aging, D-galactose, Glycoxidative stress, Learning and memory

中图分类号:

R965.2

傅永锦, 潘竞锵, 张小牧, 邝少松. 水飞蓟宾对D-半乳糖诱导衰老大鼠抑制糖氧化应激反应及改善学习记忆作用[J]. 中国临床药理学与治疗学, 2012, 17(1): 10-14.

FU Yong-jin, PAN Jing-qiang, ZHANG Xiao-mu, KUANG Shao-shong. Effects of silybin on inhibiting glycoxidative stress and improving the learning and memory in aging rats treated by D-galactose[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(1): 10-14.

参考文献

[1] 张秀丽, 刘秀珍. 梓醇对D-半乳糖致亚急性衰老小鼠学习记忆及脑中相关抗氧化酶活性的影响[J].中国生化药物杂志, 2011, 32(2):103-106.
[2] Peppa M, Uribarri J, Vlassara H.Aging and glycoxidant stress[J]. Hormones (Athens),2008, 7(2): 123- 132.
[3] Méndez JD, Xie J, Aguilar-Hernández M, et al. Molecular susceptibility to glycation and its implication in diabetes mellitus and related diseases[J]. Mol Cell Biochem, 2010,344(1/2):185-193.
[4] Tsai SJ, Chiu CP, Yang HT, et al. S-Allyl cysteine, s-ethyl cysteine, and s-propyl cysteine alleviate β-amyloid, glycative, and oxidative injury in brain of mice treated by D-galactose[J]. J Agric Food Chem,2011, 59(11):6319-6326.
[5] 李慧, 潘竞锵, 郭洁文, 等. 水飞蓟素抗肝损伤及治疗糖尿病并发症作用的研究[J]. 现代食品与药品杂志, 2007, 17(4): 18-22.
[6] 潘竞锵,吕俊华,谭海荣,等.四种中药有效成分对D-半乳糖诱导大鼠醛糖还原酶活性和蛋白非酶糖化的作用[J].中国临床康复, 2006,10(23):93-96.
[7] 王世兴,金英,李亚男,等.吡格列酮对淀粉样β蛋白片段1-42引起的大鼠学习记忆障碍及海马炎症反应的影响[J].中国药理学与毒理学杂志,2008, 22(5):348-354.
[8] 冯亮,徐辰,郑浩,等.茶多酚对D-半乳糖诱导糖基化大鼠脑损害的干预作用[J].中国老年学杂志,2008, 28(13):1251-1254.
[9] 雷鸣,朱祖健.D-半乳糖致衰老的研究进展[J].解剖科学进展, 2011,17(1):83-85.
[10] 郭长青,郭妍,陈相健,等.银杏叶提取物EGB761对D-半乳糖诱导的心肌细胞老化的保护作用[J].中国临床药理学与治疗学, 2010, 15(4):376-380.
[11] 邢秋娟,施祀,王拥军.D-半乳糖诱导衰老动物模型的机制及其在中医药方面的应用[J].上海中医药大学学报,2010,24(3): 93-98.
[12] 李文彬,韦丰,范明,等.D-半乳糖在小鼠上诱导的拟脑老化效应[J].中国药理学与毒理学杂志,1995,9(2):93-95.
[13] 宋旭,包明敏,李永明等.非酶糖基化致衰老的实验研究[J].中华老年医学杂志,1999,6(3):164-166.
[14] Tong S,Chu C,Wei Y,et al.Preparation and effects of 2,3-dehydrosilymarin, a promising and potent antioxidant and free radical scavenger[J]. J Pharm Pharmacol, 2011,63(2):238-244.
[15] Detaille D,Sanchez C,Sanz N,et al. Interrelation between the inhibition of glycolytic flux by silibinin and the lowering of mitochondrial ROS production in perifused rat hepatocytes[J].Life Sci,2008,82(21):1070-1076.
[16] 郑冬梅,陈青,郭军.醛糖还原酶抑制剂对实验性糖尿病大鼠周围神经病变的影响[J].山东医药,2008, 48(7): 37-38.
[17] 王红军,姜媛媛,路平,等.水飞蓟宾的抗肿瘤、抗氧化和免疫调节分子药理学机制研究进展[J].药学学报, 2010, 45 (4): 413-421.
[18] Lu P,Mamiya T,Lu LL,et al. Silibinin attenuates amyloid β(25-35) peptide-induced memory impairments: implication of inducible nitric-oxide synthase and tumor necrosis factor-α in mice[J]. J Pharmacol Exp Ther, 2009,331(1):319-326.
[19] Wang Q,Zou L,Liu W,et al. Inhibiting NF-κB activation and ROS production are involved in the mechanism of silibinin's protection against D-galactose-induced senescence[J]. Pharmacol Biochem Behav, 2011,98(1): 140-149.
[20] Liu B, Yang P, Ye Y, et al. Role of ROS in the protective effect of silibinin on sodium nitroprusside-induced apoptosis in rat pheochromocytoma PC12 cells[J]. Free Radic Res,2011,45(7):835-847.