Abstract:

AIM:To investigate the effects of recombinant human erythropoietin (rHuEPO) on Ang-1 during acute skin wound healing in rats. METHODS: 72 healthy male SD rats were selected to establish animal model of acute wounds, and were randomly divided into low dose group(50 U/mL rHuEPO), middle dose group(100 U/mL rHuEPO), high dose group (150 U/mL rHuEPO) and normal saline group (NS).Different doses of rHuEPO(external application) and saline were administered at the same time of modeling. Healing of the wound was observed in rats in each group. Skin samples 3 d,7 d,14 d after injury were collected to detect the changes of CD31 and Ang-1under immunohistochemistry staining. RESULTS: Compared with control group and low dose rHuEPO group,results of immunohistochemistry indicated higher expression of CD31and Ang-1 in high dose and middle dose rHuEPO group after 3 d and 7 d treatment (P<0.05). CONCLUSION: rHuEPO can increase the expression of Ang-1 and the angiogenesis of rats during healing, thereby accelerating wound healing process.

Key words: recombinant human erythropoietin, CD31, Ang-1, acute wound healing