17-DMAG inhibits cell growth and induces apoptosis of nasopharynx cancer cell HNE1

Abstract

Abstract:

AIM: To explore the effect of 17-DMAG on nasopharynx cancer cell proliferation and apoptosis. METHODS: Cell viability of HNE1 was detected by MTT assay. Cell apoptosis of HNE1 was evaluated by flow cytometry with propidium iodide staining. JC-1 staining was used to determine the effect of 17-DMAG on mitochondrial membrane potential of HNE1 cells. The expressions of Bcl-2 and Bax were detected by Western blot. RESULTS:17-DMAG inhibited the cell proliferation of HNE1 cells in a dose and time-dependent manner. PI staining revealed that 17-DMAG significantly induced HNE1 cell apoptosis（P<0.01）. JC-1 staining showed that 17-DMAG reduced the mitochondrial membrane potential of HNE1 cells. Western blot experiment showed that the expression of Bcl-2 decreased significantly (P<0.01). The expression of Bax increased significantly, especially in middle and high dose group after HNE1 cells being treated with 17-DMAG for 24 h (P<0.01). CONCLUSION: 17-DMAG inhibits the growth of HNE1 cells in a dose and time-dependent manner by inducing the apoptosis of HNE1 cells. The mechanism is related with reducing the mitochondrial membrane potential and the expression of Bcl-2, and increases the level of Bax.

Key words: 17-DMAG, nasopharyngeal cancer, cell growth, apoptosis

CLC Number:

WANG Xiu, LIU Jian, ZHANG Jingjing, ZHU Na, WANG Qingqing, LI Jianchun. 17-DMAG inhibits cell growth and induces apoptosis of nasopharynx cancer cell HNE1[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(5): 490-494.

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