Effect of sesamin on learning-memory ability and expression of glutamate in hippocampus of rats with Alzheimer's disease

Abstract

Abstract:

AIM: To study the effect of sesamin on learning-memory ability and expression of glutamate (Glu) in hippocampus of rats with Alzheimer's disease. METHODS: Forty normal SD rats were selected, ten for blank control group (NS 5.0 mL·kg^-1·d^-1). The rest were induced by intragastric administration of aluminium trichloride (AlCl3, 15 mg·kg^-1·d^-1) combined with intraperitoneal injection of D-galactose (D-gal, 180 mg·kg^-1·d^-1) for 20 weeks. After twelve weeks, rats were subsequently randomized into AD model group (NS 5.0 mL·kg^-1·d^-1), sesamin low dose group（80 mg·kg^-1·d^-1）, sesamin high dose group (160 mg·kg^-1·d^-1) (n=10). After eight consecutive weeks of drug administration, Morris water maze was used to test the learning-memory ability of rats. Immunohistochemical staining was used to determine the expression of Glu protein in hippocampal neurons. Western blot method was used to detect the expression of Glu in hippocampus. RESULTS: Sesamin can reduce the escape latency of AD rat, while increase the platform crossing numbers, improve their learning-memory ability, and reduce the positive cells and protein expression of Glu at the same time. CONCLUSION: Sesamin can reduce the expression of Glu by antagonizing Glu receptor and decrease neural excitatory toxicity, thus improving the learning-memory ability of alzheimer's disease rats.

Key words: sesamin, Alzheimer's disease, learning-memory, glutamate

CLC Number:

R965.2

WANG Huan, YANG Jieren, ZHANG Junxiu,YAO Hai, MA Tongjun. Effect of sesamin on learning-memory ability and expression of glutamate in hippocampus of rats with Alzheimer's disease[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(5): 507-511.

References

［1］Miller E, Morel A, Saso L, et al. Melatonin redox activity.Its potential clinical applications in neurodegenerative disorders［J］.Curr Top Med Chem, 2015,15(2):163-169.
［2］Reitz C. Alzheimer's disease and the amyloid cascsde hypothesis:a critical review［J］.Int J Alzheimers Dis,2012，2012:369808.
［3］邱财荣,周柏玉,吴中亮. 谷氨酸及其受体在海马学习记忆中的作用研究现状［J］.神经解剖学杂志,2016,32(4):529-531.
［4］Bournival J, Francoeur MA,Renaud J, et al. Quercetin and sesamin protect neuronal PC12 cells from high-glucose-induced oxidation, nitrosative stress, and apoptosis ［J］. Rejuvenation Res, 2012, 15(3):322-333.
［5］傅雨晨,周婷婷,汪俊,等.绿茶多酚对帕金森病模型小鼠运动和学习记忆功能的影响［J］.中国临床药理学与治疗学,2016,21(8):904-908.
［6］Kim OK,Chang JY,Nam DE,et al.Effect of canavalia gladiata extract fermented with aspergilus oryzae on the development of atopic dermatitis in NC/Nga mice［J］.Int Arch Allergy Immunol,2015,168:79-89.
［7］Lim JS, Adachi Y, Takahashi Y, et al. Comparative analysis of sesame lignans(sesamin and sesamolin)in affecting hepatic fatty acid metabolism in rats［J］. Br J Nutr，2007, 97(1):85-95.
［8］韩军,宣佳利,杨解人,等.芝麻素减轻自发性高血压大鼠肾脏纤维化作用及其机制研究［J］.中国临床药理学与治疗学,2016,21(1):16-22.
［9］Wang HM, Cheng KC, Lin CJ,et al. Obtusilactone A and (-)-sesamin induce apoptosis in human lung cancer cells by inhibiting mitochondrial Lon protease and activating DNA damage checkpoints［J］. Cancer Sci, 2010, 101(12): 2612-2620.
［10］Zhang M, Lee HJ, Park KH, et al. Modulatory effects of sesamin on dopamine biosynthesis and L-DOPA-induced cytotoxicity in PC12 cells［J］.Neuropharmacology, 2012,62(7):2219-2226.
［11］Baudry M,Bi X,Gall C,et al.The biochemistry of memory:The 26 year journey of a 'new and specific hypothesis［J］.Neurobiol Learn Mem,2011,95(2):125-133.
［12］Malleret G, Alarcon JM, Martel G, et al. Bidirectional regulation of hippocampal long-term synaptic plasticity and its influence on opposing forms of memory［J］.J Neurosci,2010,30(10):3813-3825.
［13］王杰,王蔚,虞献民,等.代谢型谷氨酸受体激动剂3,5-二羟基苯甘氨酸对大鼠学习记忆行为的影响［J］. 中国临床神经科学,2016, 24(4):371-375.
［14］Menard C, Quirion R. Group 1 metabotropic glutamate receptor function and its regulation of learning and memory in the aging brain［J］. Front Pharmacol,2012,3:182.
［15］Zempel H,Thies E,Mandelkow E,et al. Abeta oligomers cause localized Ca(2+) elevation,miss -orting of endogenous Tau into dendrites,Tau phosphorylation,and destruction of microtubules and spines［J］. J Neurosci，2010, 30( 36) : 11938-11950.

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