Abstract:

AIM: To investigate the regulation effect of deep-sea fish oil for blood lipid of rat models with hyperlipidemia and its possible mechanism. METHODS: Rats models with mixed hyperlipidemia were established by feeding high-fat diet and were divided into control group, model group and three deep-sea fish oil treatment groups (250, 500, 1 500 mg/kg), with 12 rats in each. The control group and model group received equal volume of soybean oil, while the treatment groups were administered with deep-sea fish oil for 40 days. Changes in TC,TG,HDL-C,LDL-C of serum were compared, and atherosclerosis index（AI1, AI2）,R-CHR indexes were calculated. Activities of SOD,MDA,GSH-Px in serum and liver tissue were measured by kits. Protein expression levels of SIRT1,PPAR-α were detected by Western blot. RESULTS: Compared with model group, levels of TC,TG,LDL-C in treatment groups were significantly decreased, and AI1,AI2 and R-CHR indexes in middle and high dose groups were significantly decreased. Oxidative stress was increased in hyperlipidemia rats of its serum and liver. In serum, the SOD,GSH-Px levels in high and middle dose groups were significantly increased, while the level of MDA decreased remarkably. In liver, the SOD,GSH-Px levels in middle and high dose groups were significantly increased, the level of MDA in three treatment groups decreased remarkably. Middle and high dose of deep-sea fish oil increased the protein expressions of SIRT1 and PPAR-α in liver. CONCLUSION: Deep-sea fish oil can moderate blood lipid in hyperlipidemia rats, which is probably associated with the regulation of the response level to oxidative stress in serum and liver and improving expression level of the SIRT1 and PPAR-α proteins.

Key words: deep-sea fish oil, blood lipid, antioxidation, SIRT1, PPAR-α