Protective effect of omega-3 fatty acids on hepatic fibrosis of rats induced by thioacetamide

Abstract

Abstract:

AIM: To investigate the possible protective effect of omega-3 fatty acids (n-3 FAs) from fish oil on hepatic fibrosis induced by thioacetamide (TAA) in rats. METHODS: Sixty experimental animals were divided into four groups. The control group received normal saline solution. The TAA group was given 250 mg/kg body weight of TAA. The omega-3 fatty acids group was given saline solution and supplemented with n-3 FAs. The n-3 FAs + TAA group was treated with n-3 FAs and TAA. At the end of TAA treatment for three weeks and six weeks, body weight, liver weight, and biochemical indexes were detected. HE staining was used to observe liver morphology and organizational structure. RESULTS:Treatment of rats with TAA for three and six weeks resulted in a significant decrease in body weight, while the value of liver/body weight ratio was statistically increased. Furthermore, the levels of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, gamma glutamyl transferase and total bilirubin were significantly increased. After three weeks of exposure to only TAA, liver sections showed an abnormal morphology characterized by noticeable fibrosis with the extracellular matrix collagen contents and damage of liver cells' structure. Liver sections from rats treated with only TAA for six weeks revealed an obvious increase in extracellular matrix collagen content and bridging fibrosis. Treating TAA-intoxicated rats with n-3 FAs significantly attenuated the severe physiological and histopathological changes.CONCLUSION: n-3 FAs have the antioxidation ability and can act against hepatic fibrosis induced by TAA, thus supporting its use in hepatic fibrosis therapy.

Key words: hepatic fibrosis, thioacetamide, omega-3 fatty acids

CLC Number:

R965.2
R575

ZENG Xi, HUYANG Liuzi, WU Yamin, CHEN Zhiheng. Protective effect of omega-3 fatty acids on hepatic fibrosis of rats induced by thioacetamide[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(8): 892-898.

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