Effect of antagonist of NR2A-containing N-methyl-D-aspartate receptors on synaptic long-term potentiation in hippocampus in rats with neuropathic pain

Abstract

Abstract:

AIM: To investigate the effect of antagonist of NR2A-containing N-methyl-D-aspartate (NMDA) receptors on synaptic long-term potentiation (LTP) in the hippocampus in rats with neuropathic pain. METHODS: Twenty-four adult male Wistar rats were randomly divided into four groups (n=6 each ): sham operating group (group S), S+NVP-AAM077 group (group SN), neuropathic pain group (group NP) and NP+NVP-AAM077 group (group NPN). Neuropathic pain was produced by ligation of the right L4 and L5 spinal nerve. The excitatory post-synaptic potential (EPSP) in hippocampal CA1 region was measured at 3 day after the last measurement of pain threshold. The changes in EPSP and LTP of the CA1 stratum radiatum of the rat hippocampus were observed using stereotaxic technology and extracellular recording respectively. RESULTS: The pain threshold was significantly lower in group NP and NPN, and the amplitude of EPSP was significantly higher in group NP and NPN than in group S and SN (P<0.05), but there was no significant difference in the amplitude of EPSP between group NP and NPN. CONCLUSION: The facilitation of LTP induction by neuropathic pain in the CA1 area of the hippocampus may be little relationship with the activation of NR2A-containing NMDA receptors.

Key words: neuralgia, long-term potentiation, N-methyl-D-aspartate receptors, hippocampus

CLC Number:

R441
R614

WEI Huiming, MA Weiqing, LI Wenfeng, WANG Huiming. Effect of antagonist of NR2A-containing N-methyl-D-aspartate receptors on synaptic long-term potentiation in hippocampus in rats with neuropathic pain[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(9): 1008-1011.

References

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