Welcome to Chinese Journal of Clinical Pharmacology and Therapeutics,Today is Chinese

Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2018, Vol. 23 ›› Issue (3): 329-332.doi: 10.12092/j.issn.1009-2501.2018.03.015

Previous Articles     Next Articles

Number of regulatory B cells in peripheral blood of patients with autoimmune hemolytic anemia and its clinical signifcance

HE Hesheng, JIN Xiaoke, XU Youhai, YANG Yuqiong, LIU Shanhao, QI Jing   

  1. Department of Hematology, the First Affiliated Hospital of Wannan Medical College, Wuhu 241000, Anhui, China
  • Received:2017-12-12 Revised:2018-01-20 Online:2018-03-26 Published:2018-03-28

Abstract:

AIM: To explore the number of regulatory B cells (Bregs) in peripheral blood of patients with autoimmune hemolytic anemia (AIHA) and its significance in the incidence of AIHA.  METHODS: A total of 16 AIHA patients and 14 healthy controls were enrolled. The expressions of CD19+IL-10+ Bregs and CD19+CD24hiCD27+ Bregs in peripheral blood mononuclear cells (PBMCs) were evaluated by flow cytometry. And levels of IL-10 were measured in the culture supermatants by ELISA. RESULTS: The expression of CD19+IL-10+ Bregs in PBMCs of AIHA patients was (1.27±0.39)%, which was lower than that in healthy controls (2.92±0.71)% (P<0.05). The number of CD19+CD24hiCD27+ Bregs in AIHA patients was (9.85±2.18)%, which was lower than that in healthy controls (26.47±4.31)% (P<0.05). The level of IL-10 significantly decreased in culture supermatants of autoimmune hemolytic anemia patient as compared with healthy controls (P<0.05). CONCLUSION: The expression of Bregs significantly decreases in PBMCs of AIHA patients. Bregs may play some role in the pathogenesis of AIHA.

Key words: autoimmune hemolytic anemia, regulatory B cells, interleukin-10

CLC Number: