Effects of trichostatin A on CD14+ monocytes activation and TLR4 signal pathway

doi:10.12092/j.issn.1009-2501.2018.08.005

Abstract

Abstract:

AIM: To study the effects of trichostatin A (TSA) on CD14⁺ monocytes activation and TLR4 signal pathway. METHODS: CD14⁺ monocytes were isolated from healthy donors and cultured in 1640 medium with 20 nmol/L TSA. After 24 hours, cells were harvested to detect the expression of TLR4 and downstream cytokines by RT-qPCR and flow cytometry methods. ChIP-qPCR was used to detect histone H3 acetylation level TLR4 promoter. Transwell and adhesion experiments were performed to assess the motility and adhesion ability of CD14⁺ monocytes. RESULTS: TSA treatment can increase the motility and adhesion ability of CD14⁺ monocytes. Expression of TLR4 and down-stream cytokines, including TNF-α, MCP-1 and IL-6, were up-regulated in CD14⁺ monocytes after TSA treatment. Higher acetylation of histone H3 within TLR4 promoter region was also detected in CD14⁺ monocytes after TSA treatment. CONCLUSION: TSA activates CD14⁺monocytes, promotes the expression of genes in TLR4 signal pathway and increases the histone H3 acetylation of TLR4 promoter.

Key words: trichostatin A, CD14⁺ monocytes, histone acetylation, TLR4 signal pathway, cell activation

CLC Number:

R363

WANG Yang, DU Pei, GAO Keqin, YANG Shuang, JIA Sujie. Effects of trichostatin A on CD14+ monocytes activation and TLR4 signal pathway[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(8): 867-873.

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