Chrysin attenuates oxidative stress in human umbilical vein endothelial cells

doi:10.12092/j.issn.1009-2501.2018.09.009

Abstract

Abstract:

AIM: To observe the protective effects of chrysin against H2O2-induced oxidative stress injury in human umbilical vein endothelial cells (HUVECs) and the possible mechanism. METHODS: HUVECs were subjected to H2O2 in the absence or presence of chrysin (25, 50, 100 μmol/L) for 2 h. The 3-(4,5-Dimethylthiazol-2-yl l)-2,5 -diphenyltetrazolium bromide (MTT) assay was used to assess cell viability. Reactive oxygen species was detected by dichlorofluorescein diacetate (DCFH-DA). The release of lactate dehydrogenase (LDH), the level of malondialdehyde (MDA), NO, superoxide dismutase (SOD) and endothelial nitric oxide synthase (eNOS) in culture media or in cells were assessed using commercial kit according to the manufacturer's instructions. The expressions of adhesion molecule mRNA were detected by reverse transcription-quantitative polymerase chain reaction. RESULTS:The results showed that H2O2 increased the release of LDH, the production of MDA and ROS, the mRNA level of adhesion molecules, decreased the cell viability and the activity of SOD and eNOS, reduced the production of NO. Pretreating HUVECs with chrysin improved cell viability and inhibited the release of LDH, reduced the production of ROS and MDA, alleviated the mRNA level of adhesion molecules, elevated the production of NO, improved the activity of SOD and eNOS. CONCLUSION: Chrysin might exert protective effects against oxidative stress in HUVEC.

CLC Number:

R965.2

GAO Xuemei, ZHANG Xiali, CHEN Min, LI jie, LUO Le, HAN Sijia, DONG ji, SONG Zhenrong, ZHANG Xuanping. Chrysin attenuates oxidative stress in human umbilical vein endothelial cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(9): 1022-1026.

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