Abstract:

AIM: To study the healing effect of Xianling GuBao (XLGB) plus probiotics on osteoporotic fracture rats. METHODS: Fifty female Sprague-Dawley rats were randomly divided into five groups: fracture control group, osteoporotic fracture model group, XLGB treatment group, probiotics treatment group and XLGB combined probiotics treatment group, 10 rats in each group. In addition to the conventional fracture control group, the other groups were treated with bilateral ovaries to prepare osteoporosis model. After 6 weeks, each group was prepared for right middle femoral fracture model. Then, Saline, XLGB (250 mg·kg^-1·d^-1) or probiotics (12.5 g·kg^-1·d^-1) were intragastrically administered separately. The right femur specimens of each group were taken 5 weeks after surgery. The bone mineral density was measured bone mineral density scanner, CR and Micro-CT were used to observe femur related parameters in rats. The bone alkaline phosphatase（BALP）, tartrate resistant acid phosphatase (TRACP) contents were measured by ELISA kit, calcium（Ca）concentration was measured by biochemical analyzer. HE staining was used to observe the pathological changes of periosteum in fractures. RESULTS: Compared with the osteoporotic fracture group, the BALP level was increased, the TRACP level was decreased, the bone density and the amount of callus were increased, the fracture line was blurred, the number and thickness of the trabecular bone were increased, and the trabecular space was decreased in XLGB and probiotic group, and XLGB combined probiotic group expressed the most obvious effect of treatment (P<0.05, P<0.01). CONCLUSION: Both XLGB and probiotics showed the therapeutic effect on osteoporotic fractures, and the combination of drugs has a better effect on osteoporotic fracture.

Key words: Xianling Gubao, probiotics, osteoporosis fracture healing, bone alkaline phosphatase, tartrate resistant acid phosphatase