Effects of sesamin combined with vitamin E on the cardiomyocyte apoptosis of spontaneously hypertensive rats

doi:10.12092/j.issn.1009-2501.2018.11.004

Abstract

Abstract:

AIM: To observe the inhibitory effect and mechanism of sesamin (Ses) combined with vitamin E (Vit E) on the cardiomyocyte apoptosis of spontaneously hypertensive rats (SHR). METHODS: Thirty-two spontaneously hypertensive rats (SHR) were randomly divided into SHR group, Ses combined with Vit E group (160 mg·kg^-1·d^-1+ 10 mg·kg^-1·d^-1), Vit E group (10 mg·kg^-1·d^-1), Ses group (160 mg·kg^-1·d^-1), 8 in each group. Another 8 Wistar-Kyoto rats were included as control group (0.5% CMC-Na 5 mL/kg). The animals were in tragastrically administrated with Vit E, sesamin and CMC-Na once a day for consecutive 10 weeks. After 10 weeks, the cardiomyocyte apoptosis was detected by TUNEL assay; The expression of Bax, Bcl-2 protein was detected with immunohistochemistry staining; The expression of Bax, Bcl-2, PUMA protein and the phosphorylation of p53 was detected by Western blot. RESULTS: The combination of sesamin with vitamin E significantly reduced the cardiomyocytes apoptosis rate of SHR, down-regulated the expression of Bax, Bcl-2, PUMA protein and the phosphorylation of p53, up-regulated the ratio of Bcl-2/Bax (P<0.01). CONCLUSION: Sesamin combined with vitamin E can synergistically inhibit the cardiomyocytes apoptosis of SHR, of which the potential mechanism may be related to the downregulation of PUMA protein expression and p53 phosphorylation, the regulation of the ratio of Bcl-2/Bax, and the regulation of the balance between Bax and Bcl-2 protein expression.

Key words: sesamin, vitamin E, cardiomyocyte apoptosis, Bax, Bcl-2

CLC Number:

HAO Wei, SHEN Yuanyuan, ZHANG Junxiu, YANG Jieren. Effects of sesamin combined with vitamin E on the cardiomyocyte apoptosis of spontaneously hypertensive rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(11): 1221-1227.

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