Effects of dexamethasone on the Notch signaling pathway and apoptosis-associated genes in acute pancreatitis

doi:10.12092/j.issn.1009-2501.2019.02.003

Abstract

Abstract:

AIM: To explore the effect of dexamethasone on the expression of Notch1，Hes1, Bax and Bcl-2 in pancreas in acute pancreatitis(AP). METHODS: Caerulein was used to set up AP animal model. The experimental groups were the saline group (control group), the model group (AP group) and dexamethasone group (DEX group). The content of TNF-α and IL-6 in serum in each group was detected using ELISA method. RT-qPCR was used to detect expression of Notch1, Hes1, Bax and Bcl-2 mRNA in pancreas in each group. Western blot was used to detect expression of Notch1, Hes1, Bax and Bcl-2 protein in pancreas in each group. RESULTS:The results of ELISA test showed that dexamethasone could significantly reduce the content of TNF-α and IL-6 in serum, comparing with the model group. The RT-qPCR and Western blot results showed that dexamethasone could significantly reduce the expression of Notch1, Hes1, Bcl-2 mRNA and protein in pancreas, but increase the expression of Bax mRNA and protein, as compared with the AP group. CONCLUSION: Dexamethasone could inhibit activation of the Notch signaling pathway, reduce expression of Notch1 and Hes1, further inhibit expression and antiapoptotic effect of Bcl-2 and promote expression and pro-apoptotic effect of Bax, promote apoptosis and reduce necrosis of pancreatic acinar cells, reduce the synthesis and release of TNF-α and IL-6.

Key words: acute pancreatitis, Notch signaling pathway, dexamethasone, apoptosis

CLC Number:

R657.5

KONG Lingyu, XI Zan, YANG Yaqin, MA Wenting, WU Wei, HAO Tongqin. Effects of dexamethasone on the Notch signaling pathway and apoptosis-associated genes in acute pancreatitis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(2): 134-139.

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