Study on the improvement of PI3K/AKT pathway in diabetic rats with neurogenic bladder disease by extract of semen plantannical

doi:10.12092/j.issn.1009-2501.2019.11.006

Abstract

Abstract:

AIM: To investigate the effects of semen plantain extract on the improvement of neurogenic bladder disease in diabetic rats and the PI3K/AKT pathway. METHODS: Fifty SD rats of the same weight and age were randomly divided into 5 groups, the control group, model group, low dose group, middle dose group and high dose group, 10 in each group. Model group and dosage groups were built of diabetic neurogenic bladder disease model. The blood glucose, food intake, urine volume change, water quantity, and weight were recorded and observed after 4 weeks. After the completion of the model, the low-dose, medium-dose and high-dose groups were treated with plantago extract at the corresponding concentrations from low to high respectively, and the changes in blood glucose from week 5 to 9, metabolism indexes from week 8 and 9, and urodynamic indexes from week 9 were observed. Fluorescence quantitative PCR and Western blot were used to detect the expression of genes related to the PI3K/AKT pathway. RESULTS:Compared with the control group, the blood glucose level in the model group was higher than that in the control group from the first week after modeling (P<0.05), and the blood glucose level in the following three weeks was still higher than those in the control group (P<0.05). At 3th week and 4th week, the food and water intake and urine in the model group was higher than those in the control group (P<0.05), and the body weight of 1st week was lower than that in the control group (P<0.05). At 9th week, the blood glucose level of the middle and high dose groups returned to normal level, and no statistical difference was found between the two groups (P>0.05). The blood glucose level of the high dose group was close to normal level in terms of food and water intake, urine volume and urodynamics, and no statistical difference was observed between the two groups (P>0.05). Compared with the control group, the expressions of PI3K, AKT, p-PI3K and p-AKT in the model group, the low-dose group and the middle-dose group were all significantly decreased (P<0.05), while the differences between the high-dose group and the control group were not significant (P>0.05). CONCLUSION: A certain dose of plantain seed extract can regulate the expression of precursors in the PI3K/AKT pathway, and has significant improvement on neurogenic bladder disease in diabetic rats.

Key words: semen plantaginis extract, PI3K/AKT pathway, diabetic rats, neurogenic bladder disease

CLC Number:

ZHANG Heng, WU Haixiao, HU Yang, YANG Qing, HUANG Ting. Study on the improvement of PI3K/AKT pathway in diabetic rats with neurogenic bladder disease by extract of semen plantannical[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(11): 1249-1255.

References

［1］Divino V, Boye KS, Lebrec J, et al. GLP-1 RA treatment and dosing patterns among type 2 diabetes patients in six countries: A retrospective analysis of pharmacy claims data［J］. Diabetes Ther, 2019,10(3):1067-1088.
［2］Ohmura H, Mita T, Matsuoka J, et al. Real-world data on the incidence of macrovascular complications in Japanese patients with type 2 diabetes: The sitagliptin registration type 2 diabetesjuntendo collaborating project［J］. Diabetes Ther, 2019,10(3):1099-1011.
［3］Takemura K, Takazawa R, Kohno Y, et al. Vesical fungus balls (fungal bezoars) by Candida albicans mimicking urothelial carcinoma in a patient with diabetic neurogenic bladder［J］. Urol Case Rep, 2018, 18:50-51.
［4］Niu X, Wang X, Huang H, et al. Bulbocavernosus reflex test for diagnosis of pudendal nerve injury in female patients with diabetic neurogenic bladder［J］. Aging Dis, 2016,7(6):715-720.
［5］Nayar S, Pawar B, Einsiedel L, et al. Isolated neurogenic bladder associated with human T-lymphotropic virus type 1 infection in a renal transplant patient from central Australia: A case report［J］.Transplant Proc, 2018, 50(10):3940-3942.
［6］Fukuda T, Bouchi R, Minami I, et al. Retrograde pyelonephritis and lumbar spondylitis as a result of Salmonella typhi in a type 2 diabetes patient with neurogenic bladder［J］. J Diabetes Investig, 2016, 7(3):436-439.
［7］李冲冲, 龚苏晓, 许浚, 等. 车前子化学成分与药理作用研究进展及质量标志物预测分析［J］. 中草药, 2018,49 (6):1233-1246.
［8］张居卫, 张建顺, 张海英. 车前子醇提取物对不同糖尿病模型小鼠降血糖效果的评价［J］.中国基层医药, 2015, (12):32-33.
［9］Vernstrom L, Laugesen E, Grove EL,et al. Differential vascular effects of aspirin in people with Type 2 diabetes without cardiovascular disease and matched controls without diabetes［J］. Diabet Med, 2019. doi: 10.1111/dme.13978.
［10］Gianatti EJ, Grossmann M. Testosterone deficiency in men with Type 2 diabetes: pathophysiology and treatment［J］. Diabet Med, 2019. doi: 10.1111/dme.13977.
［11］Hertroijs DFL, Elissen AMJ, Brouwers MCGJ,et al. Preferences of people with Type 2 diabetes for diabetes care: a discrete choice experiment［J］. Diabet Med, 2019, 29(7):869-877.
［12］Hulls CM, Lentle RG, King QM, et al. Pharmacological modulation of the spatiotemporal disposition of micromotions in the intact resting urinary bladder of the rabbit; their pattern is under both myogenic and autonomic control［J］. BJU Int, 2019,123:S54-64.
［13］Wade D, Cooper J, Derry F, et al. Uro-Vaxom versus placebo for the prevention of recurrent symptomatic urinary tract infections in participants with chronic neurogenic bladder dysfunction: a randomised controlled feasibility study［J］. Trial,2019, 20(1):223.
［14］Bragge P, Guy S, Boulet M,et al. A systematic review of the content and quality of clinical practice guidelines for management of the neurogenic bladder following spinal cord injury［J］. Spinal Cord, 2019.doi: 10.1038/s41393-019-0278-0.
［15］Kang SH, Oh SJ, Jeong SJ, et al. Linguistic validation of the intermittent self-catheterization questionnaire for patients with neurogenic bladder who perform intermittent catheterization for voiding dysfunction［J］. Int Neurourol J, 2019, 23(1):75-85.
［16］Magarifuchi H, Kusaba K, Yamakuchi H, et al. Staphylococcus saprophyticus native valve endocarditis in a diabetic patient with neurogenic bladder: A case report［J］. J Infect Chemother, 2015, 21(9):695-699.
［17］Powell CR. Is the diabetic bladder a neurogenic bladder? Evidence from the literature［J］. Curr Bladder Dysfunct Rep, 2014, 9(4):261-267.
［18］张然, 袁从英, 冯娜, 等. 车前子多糖对糖尿病小鼠氧化应激的影响［J］. 天津医药，2011，39(3): 253-255.
［19］Chen L, Gong HY, Xu L. PVT1 protects diabetic peripheral neuropathy via PI3K/AKT pathway［J］. Eur Rev Med Pharmacol Sci, 2018, 22(20):6905-6911.
［20］Wang W, Li P, Xu J, et al. Resveratrol attenuates high glucose-induced nucleus pulposus cell apoptosis and senescence through activating the ROS-mediated PI3K/Akt pathway［J］. Biosci Rep, 2018, 38(2). pii: BSR20171454. doi: 10.1042/BSR20171454.

[1]	XIONG Fei, SHI Yanyan, SHEN Jiucheng, CHENG Honglan, HANG Yongfu. Inhibitory effect and mechanism of cinobufotalin on lung cancer NCI-A549 xenograft in nude mice [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(10): 1103-1108.
[2]	WEN Zhi-Ming. Influence of different dosages and injecting times of streptozotocin on islets of diabetic rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(10): 1128-1133.
[3]	WEN Zhi-Ming. Effects of 3 traditional Chinese formulas of herbal medicine inPrevention and treatment of diabetic rats induced by streptozotocin [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2003, 8(6): 680-685.