Mechanism of total flavonoids of Rhododendron blocking Rho kinase-induced vasodilation of coronary artery in rats

doi:10.12092/j.issn.1009-2501.2019.12.006

Abstract

Abstract:

AIM: To investigate the mechanism of total flavonoids of Rhododendron blocking Rho kinase-induced vasodilation of coronary artery in rats. METHODS: ROCK1-siRNA and ROCK2-siRNA rats were prepared by siRNA technique. ROCK1 and ROCK2 of coronary artery were measured by western-blot. Coronary artery tension was measured by a microvascular tension system. The diastolic function of normal and interfered vascular was detected with Y27632 and TFR. RESULTS:Compared with control group, the expression of ROCK1in ROCK1-siRNA group and expression of ROCK2 in ROCK2-siRNA group were decreased, while the expression of ROCK1 and ROCK2 in negative siRNA and transfection reagent groups were not significant difference, siRNA interference technology can effectively reduce expression of ROCK1 and ROCK2 of coronary artery tissue.Compared with vehicle group, TFR and Y27632 can cause vasodilation in ROCK1-siRNA and ROCK2-siRNA transfected vascular. Compared with normal group, TFR and Y27632 significantly attenuated the vasodilation in ROCK1-siRNA and ROCK2-siRNA transfected vascular; The Emax of vasodilation which induced by TFR in ROCK2-siRNA group was higher than ROCK1-siRNA group.CONCLUSION: The vasodilation of coronary artery which induced by total flavonoids of Rhododendron on rats is associated with blocking ROCK and may selectively act on ROCK1.

Key words: total flavonoids of Rhododendron, coronary artery, vasodilation, Rho-associated kinase

CLC Number:

R965.2
R543

LI Yanan, CHEN Zhiwu, GUO Yan. Mechanism of total flavonoids of Rhododendron blocking Rho kinase-induced vasodilation of coronary artery in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(12): 1353-1357.

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