Abstract: The physiologically based pharmacokinetic (PBPK) model, as a physiological mechanism-based model, can simulate the absorption, distribution, metabolism and excretion of drugs in the body. In recent years, it has been widely used in drug-drug interaction study, special population extrapolation, clinical trial dose selection, individualized medication and impact of different factors on the pharmacokinetic course. This review briefly describes the history and current development of PBPK model, introduces the work flow of PBPK model, and reviews its application in drug clinical study in recent years. We hope this review could provide a reference for researchers who are interested in this field.