Abstract: AIM: To prepare genistein combined polysaccharide (GCP) liposomes and to evaluate the potential value of GCP liposomes for the treatment of prostate cancer by in vivo and in vitro experiments, and ultimately to promote the application of nanotechnology in prostate cancer chemotherapy. METHODS: GCP liposomes were prepared by ethanol injection. The anti-tumor effect of GCP liposomes was verified in vitro and in vivo by cell proliferation assay and cell cycle analysis, as well as establishing a tumor model of prostate cancer.RESULTS:GCP liposomes enhanced the inhibitory effect of GCP on androgen-sensitive LNCaP cell proliferation and the apoptosis of androgen-sensitive LNCaP cells induced by GCP. Also, GCP liposomes improved the inhibitory effect of GCP on tumor growth in tumor-bearing mice. CONCLUSION: GCP liposomes have better anti-tumor effect on androgen-sensitive human prostate cancer cells than GCP.

Key words: genistein combined polysaccharide, liposomes, prostate cancer