Effect of Urotensin II on the apoptosis of human lung adenocarcinoma A549 cells and the underlying mechanism

Abstract

Abstract: AIM: To explore the effect of Urotensin II on the apoptosis of human lung adenocarcinoma A549 cells and its underlying mechnism. METHODS: A549 cells were divided into three groups: Control group, Cisplatin group (25 μg/mL)and Urotensin II+Cisplatin group(10^-7 mol/L U-Ⅱ+ 25 μg/mL cisplatin). The effect of Urotensin II on the apoptosis of A549 cells was determined by TUNEL method. The expressions of Bcl-2 and Bax were detected by immunocytochemistry. RESULTS: Comparing with Control group, the apoptotic rate and the expression of Bax were increased, while the expression of Bcl-2 was decreased in Cisplatin group(P<0.01). Urotensin II significantly inhibited Cisplatin-induced apoptosis of A549 cells and antagonized Cisplatin-induced down-regulation of Bcl-2 and up-regulation of Bax(P<0.01). CONCLUSION: Urotensin II inhibits the apoptosis of human lung adenocarcinoma A549 cells by up-regulating the expression of Bcl-2 and down-regulating the expression of Bax.

Key words: Urotensin II, Lung adenocarcinoma, A549, Apoptosis, Bcl-2, Bax

CLC Number:

R734

ZHOU Cheng-hua, SONG Zheng, XING Shu-hua, WU Yu-qing, ZHENG Jun-nian, PEI Dong-shang. Effect of Urotensin II on the apoptosis of human lung adenocarcinoma A549 cells and the underlying mechanism[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(7): 742-746.

References

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