Effects of crocetin on the proliferation and differentiation of 3T3-L1 preadipocyte

Abstract

Abstract: AIM: To observe the effect of crocetin on proliferation and differentiation of 3T3-L1 preadipocyte, and to investigate the possible mechanism involved. METHODS: The 3T3-L1 cells were pretreated with crocetin, while, the MTT method was used to detect the proliferation of the cells, oil red O staining method and spectrophotography were applied to analyze the degree of differentiation. The expression of AMP-activated protein kinase(AMPK)mRNA, which was called as the energy regulator of cell, was detected by reverse transcription PCR (RT-PCR), and the protein level of p-AMPK was determined by western blot. RESULTS: High and middle dose crocetin(10^-5, 10^-6 mol/L) could significantly inhibit the proliferation and differentiation of 3T3-L1 cell(P＜0.01); also, the low dose croctin(10^-7 mol/L) could inhibit its proliferation and differentiation(P＜0.05); croctin could incease the expression of AMPK mRNA and the protein level of p-AMPK(P＜0.05). CONCLUSION: Crocetin inhibit the proliferation and differentiation of 3T3-L1 cell possibly by influence AMPK.

Key words: Crocetin, 3T3-L1 cell, Proliferation and differentiation, AMP-activated protein kinase

CLC Number:

R965.2

ZHANG Xiao-ming, QIAN Zhi-yu, JI Hui, YANG Li-na, YANG Ru-hui, SONG Lei. Effects of crocetin on the proliferation and differentiation of 3T3-L1 preadipocyte[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(7): 753-757.

References

[1] 刘彩艳,魏建. 肥胖与胰岛素抵抗及2型糖尿病的关系[J]. 实用医药杂志, 2004,21(6):558.
[2] 彭飞城,皋聪,钱之玉. 西红花酸对小鼠缺氧损伤的保护作用[J]. 中国新药杂志, 2007,16(21):1772-1775.
[3] 盛亮,钱之玉,季晖. 西红花酸改善大鼠高三酰甘油血症的作用机制[J]. 中国新药杂志, 2008,17(21): 1843-1847.
[4] He SY, Qian ZY, Tang FT, et al. Effect of crocin on experimental atherosclerosis in quails and its mechanisms[J]. Life Sci, 2005,77(8):907-921.
[5] 赵博华,钱之玉,杨丽娜,等. 西红花酸对大鼠血浆胆固醇的调节作用及其机制研究[J]. 中国新药杂志, 2009,18(15):45-49.
[6] Xi L, Qian ZY, Xu GL, et al. Beneficial impact of crocetin, a carotenoid from saffron, on insulin sensitivity in fructose-fed rats[J]. J Nutr Bioche, 2007,18(1):64-72.
[7] Xi L, Qian ZY. Pharmacological properties of crocetin and crocin(digentiobiosyl ester of crocetin) from saffron[J]. Nat Prod Commun, 2006,1(1):66-68.
[8] 刘华,曹郁生. 共轭亚油酸对前脂肪细胞增殖分化的影响[J]. 天然产物研究与开发, 2008,20(1):8-11.
[9] Lu HL, Wang HW, Cianflone K, et al. Acylation stimulating protein inducing 3T3-L1 preadipocyte differentiation[J], Chin J Biochem Mol Biol, 2004,20(1):383-386.
[10] Choi JS, Jung MH, Park HS, et al. Effect of conjugated linoleic acid isomers on insulin resistance and mRNA levels of genes regulating energy metabolism in high-fat-fed rats[J]. Nutrition, 2004,20(11/12):1008-1017.
[11] Huypens P, Quartier E, Pipeleers D, et al. Metformin reduces adiponectin protein expression and release in 3T3-L1 adipocytes involving activation of AMP activated protein kinase[J]. Eur J Pharmacol, 2005,518(23):90-95.
[12] 巫冠中,郭永起,苏欣,等. 大豆黄素衍生物LRXH609 的减肥作用及机理探讨[J]. 中国临床药理学与治疗学, 2009,14(5):519-513.
[13] 戴冰,肖子曾,刘磊,等. 六味地黄丸入血成分莫诺苷对大鼠前脂肪细胞增殖与分化的影响[J]. 中国临床药理学与治疗学, 2007,12(11):1245-1249.
[14] 徐静,刘毅,完强,等. 高糖环境对大鼠肾小球系膜细胞AMPK表达及活性的影响[J]. 山东大学学报, 2009,47(5):46-49.
[15] Zhou Y, Wang D, Zhu Q, et al. Inhibitory effects of A-769662, a novel activator of AMP-activated protein kinase, on 3T3-L1 adipogenesis[J]. Biol Pharm Bull, 2009,32(6):993-998.
[16] Kong CS, Kim JA, Kim SK. Anti-obesity effect of sulfated glucosamine by AMPK signal pathway in 3T3-L1 adipocytes[J]. Food Chem Toxicol, 2009, 47(10):2401-2406.