Abstract: AIM: To evaluate the sustained-release effect on Compound Danshen Skeleton Sustained-Release Tablet (CDSRT) by pharmacodynamics method.METHODS: By reference to Compound Danshen Tablet(CDT), Sprague-Dawley rats had been administrated with CDT and CDSRT with the content of crude drugs 2911.14 mg/kg. The medicated serum of CDT and CDSRT were prepared at the time of 0.25,0.5,0.75,1,2,4,8,12 h after single oral administration. The medicated serums were added to myocardial cells respectively. The protective effects of CDT and CDSRT on hypoxia/reoxygenation and the sustained-release effect of CDSRT were evaluated.RESULTS: Compared with the H/R model group, the medicated serum of CDT 4,8 h and CDSRT 2,4,8,12 h had apparent protective effects against proliferation inhibition caused by hypoxia/reoxygenation(H/R) induced injury in myocardial cells. The present half-life t_1/2(ka) of CDT and CDSRT were 2.655 h and 0.719 h. The peak times of effective action were 5.213 h and 2.957 h. And the elimination half-life t_1/2(ke) were 4.953 h and 10.166 h respectively. Compared with CDT, CDSRT had a higher speed of the effect present and the peak time of effective action and a lower speed of sustained-release.CONCLUSION: CDSRT has a certain sustained-release function as t_1/2(ke) longer than that of CDT. Pharmacodynamics method is a feasible method for the evaluation of CDSRT sustained-release effect.

Key words: Compound Danshen Skeleton Sustained Release Tablet, Pharmacodynamics, Sustained-release function