Abstract: AIM: To study the anti-bradycardic effect of ganfu granule (Gan) in rats. METHODS: The bradycardic model in rats was induced by propranolol (Pro) or acetylcholine (ACh).The content of malonaldehyde (MDA) was measured by TBA method, the Green method was used to determine the content of nitric oxide (NO). RESULTS: Gan(30, 90, 270mg/kg) significantly inhibited the Pro-induced decrease of heart rate in rats, and shortened the time of rat bradycardia induced by ACh. Gan 30, 90, 270mg/kg markedly increased the NO content, and reduced the MDA content in rat myocardium. CONCLUSION: Gan has the obvious anti-bradycardic effect via increasing NO reduction, while attenuating oxygen free radical damage.

Key words: ganfu granule, bradycardia, nitric oxide