Effect of extracellular signal-regulated kinase on apoptosis of pneumocyte during lung ischemic and reperfusion injury in rats

Abstract

Abstract: AIM: To explore the effect of extracellular signal-regulated kinase (ERK) on apoptosis of pneumocyte during lung ischemic and reperfusion injury in rats. METHODS: 30 rats were randomly divided into three groups:control group(Group C), lung ischemia- reperfusion injury group(Group I R) and PD98059 group(Group P).The content of MDA in serum, the activity of SOD, cell apoptosis index and the p-ERK protein expression were detected by immunohistochemistry, and the ultrastruction changes of lung were observed by electron microscope. RESULTS: Compared with Group C, the content of MDA was increased, the content of SOD was decreased remarkably (all P < 0.01), the expression of p-ERK protein was increased remarkably (P <0.01), and the apoptosis index (AI) was increased in Group I R (P <0.01), abnormal changes of the lung tissue in morphology were observed in Group I R.After PD98059 treatment, the content of MDA was increased (P <0.01), the content of SOD was decreased (P <0.05), the expression of p-ERK protein was decreased(P <0.01), the value of AI was increased(P <0.01), the abnormal changes of the lung tissue in morphology were aggravated markedly. CONCLUSION: The ERK signal transduction pathway maybe protect the lung from the injury caused by ischemia and reperfusion through lessening apoptosis of lung cells in rats.

Key words: lung, reperfusion injury, erk, PD98059, apoptosis

CLC Number:

R363

HONG Jia-lin, ZHENG Yan-rong, SHI Lu, JIA Xu-guang, WANG Wan-tie. Effect of extracellular signal-regulated kinase on apoptosis of pneumocyte during lung ischemic and reperfusion injury in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(10): 1137-1141.

References

[1] Sekido N, Mukadia N, Harada A, et al.Prevention of lung reperfusion injury in rabbits by a monoclonal antibody against interleukin-8[J].Nature, 1993, 365(6447):654.
[2] Weber NC, Stursberg J, Wirthle NM, et al.Xenon preconditioning differently regulates p44 42MAPK (ERK1 2) and p46 54MAPK (JNK1 2 and 3) in vivo[J].British Journal of Anaesthesia, 2006, 97(3):298-306.
[3] 段红, 李源, 王鲜平, 等.川芎嗪对缺血再灌注大鼠心肌细胞凋亡的影响[J].武警医学, 2000, 11(2):70-73.
[4] Ng CS, Wan S, Yim AP.Pulmonary ischaemia-reperfusion injury:role of apoptosis[J].Eur Respir J, 2005, 25(2):356-363.
[5] Gary L.The protein kinase complement of the human genome[J].Science, 2002, 298(5600):1912-1934.
[6] Chang L, Karin M.Mammalian MAP kinase signalling cascades[J].Nature, 2001, 410(6824):37-40.
[7] Ballard-Croft C, Locklar AC, Kristo G, et al.Regional myocardial ischemia-induced activation of MAPKs is associated with subcellular redistribution of caveolin and cholesterol[J].Am J Physiol Heart Circ Physiol, 2006, 291(2):H658-667.
[8] Thorburn J, CarlsonM, Mansour SJ, et al.Inhibition of a signaling pathway in cardiac muscle cells by active mitogen- activated protein kinase kinase [J].MolBiol Cell, 1995, 6(11):1479-1490.
[9] Widmann C, Gibosn S, JarpeMB, et al.Mitogen-activated protein kinase:conservation of a three-kinase module from yeast to human [J].Physiol Rev, 1999, 79(1): 143-180.
[10] 吴献伟, 吴丽, 薛荣亮.全脑缺血再灌注后磷酸化ERK 蛋白的表达及应用PD98059 后对其表达的影响[J].实用医技杂志, 2008, 15(2):148-150.
[10] 李军, 曹红, 连庆泉, 等.ERK 通路在脑缺血及缺血预处理沙土鼠海马神经元中的作用[J].中国应用生理学杂志, 2008, 24(2):237-242.
[11] 武蓓, 陈红, 任景宜, 等.心肌缺血再灌注损伤中细胞凋亡的信号转导通路[J].中国医药导刊, 2007, 5(9):364-367.

[1]	YOU Rongli, HUANG Yurong, MAORui, HAI Lina, WANG Yingli, WANG Yan. Mechanism of compound kushen injection in the treatment of lung cancer based on serum metabolomics and network pharmacology [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(9): 988-999.
[2]	FAN Jie, JIN Yongming, JIANG Xiaolong, JIANG Guohua. Molecular mechanism of lncRNA HOTAIR regulating miR-206 on the proliferation and apoptosis of rheumatoid arthritis synovial cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(7): 736-742.
[3]	HOU Qiong, LIU Fei, CHEN Chuanrong. Immunotherapy combined with anti-angiogenic drugs and chemotherapy in negative driver gene and advanced non-small cell lung cancer [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(7): 775-779.
[4]	FU Ting, LI Fang, PAN Dayan, XIA Yuanyuan, ZHANG Qiuyuan. miR-718 from exosomes of lung cancer cells induces angiogenesis by targeting PTEN [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(6): 624-632.
[5]	YANG Xiaojia, JIANG Meng, WU Min, ZHANG Yili, LV Lan, WU Yuanfen, WANG Xinyu, LIU Liquan. Improvement effect of crocin on cognitive impairment of Alzheimer's disease rats through DKK3 regulation of GSK-3 β/β-Catenin pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(5): 489-497.
[6]	QI Xiaofeng, LUO Yali, XIAO Mengyong, ZHOU Shiqin, ZHOU Wen, AN Fangyu, WEI Benjun, LIU Yongqi. Research status of dialectical prevention and treatment of acute lung injury based on the theory of "Wei qi and Ying Xue" [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(4): 429-437.
[7]	ZHANG Xuejiao, LIU Jieting, LI Luxin, CHEN Peijian, DING Minglu, SUN Mengwei, CHU Yanhui, ZHANG Zhen . Effects and mechanism of dapagliflozin on myocardial injury in type 1 diabetes mice [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(3): 257-265.
[8]	HE Yang, YANG Kui, LUAN Jiajie. Effects of CDA-G208A gene polymorphism on the efficacy of gem-citabine in the first-line treatment of lung squamous cell carcinoma [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(3): 299-306.
[9]	HOU Xiaoyu, LENG Yufang, CAO Xuefen, LV Xingjiao, HAN Xiaoxia, Janvier NIBARUTA, LIU Yongqiang. Network analysis and experimental verification of Schisandrin B reduces intestinal ischemia reperfusion injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(2): 147-154.
[10]	ZHANG Lei, XIAO Xingpeng, DING Huang . Silencing ZKSCAN3 gene expression aggravates lung injury induced by LPS in mice [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(2): 164-170.
[11]	WANG Jian, SUN Yongdong, ZHOU Xingwei, LIU Lei, CHEN Long, TONG Xingke, ZHU Jiali. Baicalein promotes laryngeal cancer cell death and inhibits invasion via miR-125b-5p/IRF4 axis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(11): 1209-1218.
[12]	ZHU Ruifang, GUO Dongkai, ZHI Hui, JIANG Yiguo, ZHANG Yueling, QIAN Xiaoping, JI Shiliang. Exploring the mechanism of elemene synergistic bortezomib against multiple myeloma by ROS-NF-κB-p38MAPK pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(11): 1219-1226.
[13]	LI Fei, DING Huiqin, CHEN Mengjing. Isonlosinine induces autophagy of PC9 cells by ERK signaling pathway in lung cancer [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(11): 1235-1240.
[14]	LIU Jiajun, QUE Zujun, TIAN Jianhui. Comparative study on the ability of active ingredients in Jinfukang to decrease neutrophils recruiting by lung cancer circulating tumor cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(1): 1-9.
[15]	SU Yang, ZHOU Feng, DING Jinlei. Effects of esketamine combined with sufentanil on postoperative analgesia and emotion after thoracoscopic radical resection of lung cancer [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(1): 59-65.