Protective effect of preparation of nanometer tanshinone ⅡA against global cerebral ischemia-reperfusion injury in rats

Abstract

Abstract: AIM: To investigate the protection effect of preparation of nanometer Tanshinone ⅡA against brain injury in rats undergone global cerebral ischemia reperfusion.METHODS: The rats were intragastrically administrated with the preparation of nanometer Tanshinone ⅡA , then the global cerebral ischemia-reperfusion model of rat was made by clipping and releasing three arteries.The content of MDA and the activity of SOD and Na⁺-K⁺-ATPase in homogenate of the cerebral tissues were measured.The sections of pathology and ultrastructure of mitochondrion in cortex had been observed.RESULTS: Tanshinone ⅡA (25 mg/kg ) could obviously decrease the content of MDA (P<0.01)and increase the activity of SOD(P<0.05) and Na⁺-K⁺-ATPase(P<0.05)in rats brain tissue.The observation of pathology and ultrastructure showed that the injury of cell and mitochondrion had been restrained in these of rat brain.CONCLUSION: Preparation of nanometer Tanshinone ⅡA has protective effect against global cerebra injury caused by global cerebral ischemia-reperfusion injury in rats.

Key words: preparation of nanometer tanshinone ⅡA, global cerebral ischemia-reperfusion injury, free radicals, Na⁺-K⁺-ATPase, ultrastructure of cell

CLC Number:

R965.2

CHEN Feng, YE Long-bin, XIN Zhong-suai, XI Tao. Protective effect of preparation of nanometer tanshinone ⅡA against global cerebral ischemia-reperfusion injury in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(4): 391-396.

References

[1] 徐任生.丹参-生物学及其应用[M].北京:科学出版社, 1990:6.
[2] 郑志远, 黄文起, 卢振和.丹参酮ⅡA 对兔脑缺血再灌注损伤的保护作用[J].中西医结合心脑血管病杂志, 2005, 3(12):1066-1068.
[3] Lam BYH, Lo ACY, Sun XR, et al.Neuroprotective effects of tanshinones in transient focal cerebral ischemia in mice[J].Phytomedicine, 2003, 10(4):286-291.
[4] 罗厚蔚, 陈峰.中国专利, 02137863.0[P].
[5] 陈峰, 罗厚蔚.应用固体分散技术制备丹参酮胶体制剂[J].电子显微学报, 2007, 26(2):148-152.
[6] 叶龙彬, 奚涛, 陈峰.丹参酮ⅡA 对大鼠局灶性脑缺血再灌注损伤的保护作用[J].中国药科大学学报,2004, 35(3):267-270.
[7] KameyamaM, Suzuki J, Shirane R, et al.A new model of bilateral hemispheric ischemia in the rat three vessel occlusion model[J].Stroke, 1985, 16(3):489-493.
[8] Salim S, AhmadM.Protective effect of Nardostachys jatamansi in rat cerebral ischemia[J].Pharmacol Biochem Behav, 2003, 74(2):481-486.
[9] 王治军, 许幸, 吴新民.地塞米松、甘露醇对大鼠全脑缺血再灌注损伤的治疗作用[J].北京大学学报:医学版, 2003, 35(3):303-306.
[10] Traughler JM, Hall ED.Central nervous system trauma and stoke:I.Biochemical considerations for oxygen radical formation and lipid peroxidation[J].Free Radic Biol Med, 1989, 6(3):289-301.
[11] Dimagl U, Lindauer U, Them A.Global cerebral ischemia in the rat:online monitoring of oxygen free radical production using chemiluminescence in vivo[J].J Cereb Blood Flow Metab, 1995, 15(6):925-940.
[12] McCord JM.Oxygen-derived free radicals in post ischemic tissue injury[J].N Engl Med, 1985, 312:159-163.
[13] Oliver CN, Starke-Reed PE, Stadtman ER, et al.Oxidative damage to brain proteins, loss of glutamine synthesis activity, and production of free radicals during ischemia reperf-usion-induced injury to gerbil brain[J].Proc Natl Acad Sci USA, 1990, 87(13):5144-5147.
[14] Traystman RJ, Kirsch JR, Koehler RC.Oxygen radical 2009 Apr;14(4)mechanism of brain injury following ischemia-reperfusion [J].J Appl Physiol, 1991, 71(4):1185-1195.
[15] 朱松君, 朱晓峰.ATP 酶与脑缺血及缺血再灌注损伤[J].黑龙江医药科学, 1999, 22(6):58.
[16] Allen KL, Almeida A, Bates TE, et al.Changes of respiratory chain activity in mitochondrial and synap tosomal fractions isolated from the gerbil brain after graded ischemia[J].Neurochem, 1995, 64(5):2222-2229.
[17] Almeida A, Allen KL, Bates TE, et al.Effect of reperfusion following cerebral ischemia on the activity of the mitochondrial resp iratory chain in the gerbil brain[J].Neurochem, 1995, 65(4):1698-1703.
[18] 韩济生.神经科学纲要[M].北京:北京医科大学, 中国协和医科大学联合出版社, 1993:32-38.
[19] 成同怡.Na +-K+-ATPase 的结构和功能[J].国外医学分子生物学分册, 1990, 12(5):56-58.
[20] Stojanoric T, Mrsul ja BB.Alteration in synaptos membrane Na⁺-K⁺-ATPase of the ferbil cortex and hippocampus following reversible brain ischemia[J].MeTab Brain Dis, 1988, 13(4):256-261.