Role of cytochrome P450 on the metabolism of injection bupleuri

Abstract

Abstract: AIM: To identify the effects of injection bupleuri.on activity of CYPs subgroups.METHODS: The activity of CYP1A2 was determined by measuring the ratio of caffeine and its metabolite in whole blood in vivo by HPLC and the ratio of phenacetin and its metabolite in liver microsomes in vitro.The expression of CYP1A2、 3A4、4A1 in three groups were examined by Western blotting.RESULTS: There were no significant difference between probes metabolic rates among groups in vivo and in vitro(P>0.05).Also there were similar expression of CYP1A2 in rat liver microsomes from control and treated groups.The expression of CYP3A4 increased in the high dose bupleuri.group.the expression of CYP4A1 obviously decreased in bupleuri.groups.CONCLUSION: Injection bupleuri can inhibit CYP4A1 and induce CYP3A4 expression in rat livers.however it does not effect rat CYP1A2 in vivo or in vitro obviously.

Key words: cytochrome P450, probe, injection bupleuri, liver microsome

CLC Number:

R965.2

WANG Jing, YU Hui-yan, GUO Lei, LIU Yan, ZHU Da-ling. Role of cytochrome P450 on the metabolism of injection bupleuri[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(9): 1037-1041.

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