Methodological study on improving tumor formation rate of xenografted human lung cancer in nude mice

Abstract

Abstract: AIM: To improve tumor formation rate of xenografted human lung cancer in nude mice.METHODS: Before xenograftion, human lung cancer SPC-A-1 or GLC-82 cells and their tissue homogenate were diluted with supernatant of mice sarcoma 180 ascites being rich in growth factors.The diluted SPC-A-1 or GLC-82 cell suspensions were injected subcutaneously at right back or armpit of nude mice.The effects of supernatant of mice sarcoma 180 ascites on the tumor growth rate and tumor formation rate were evaluated.RESULTS: Compared with control group, the tumor growth rate and tumor formation rate of human lung cancer SPC-A-1 or GLC-82 in nude mice were significantly improved with the addition of the supernatant of mice sarcoma 180 ascites.CONCLUSION: The supernatant of mice sarcoma 180 ascites can significantly improve the tumor growth rate and tumor formation rate of human lung cancer SPC-A-1 or GLC-82 in nude mice.

Key words: human lung cancer, nude mice, tumor model, inhibition rate

CLC Number:

R965.2

ZHANG Bao-lai, XU Ming-li, GAO Ming-tang, LU Li, WANG Li, CUI Ming-xia, LI Wen-guang, WU Yong-jie. Methodological study on improving tumor formation rate of xenografted human lung cancer in nude mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(9): 1052-1057.

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