Abstract: AIM: To establish an HPLC mehod for the analysis of pharmacokinetics of salvianolic acid B in rats. METHODS: The biological samples were extracted with acetic ether. The chromatographic conditions were as follows: Hypersil ODS column (200 mm ×4.6 mm,5μm)was used. The mobile phase was acetonitrile-water (with Ammoniom Acetate 0.25 mol/L)(25:75,v/v) at a flow-rate of 1.0 mL min,and the detection wave length was set at 328 nm. RESULTS: Salvianolic acid B was injected intravenously at doses of 1.6,3.2,6.4 mg/kg. The terminal elimination half-life(t 1 2)of αphase and β phase was (3.1±0.1)min and (31.5±3.2) min. The extents of excrement,urine and biliary excretion of salvianolic acid B were 1.43 %±0.90 %, 0.77 %±1.01 % and 8.82 %±4.11 %.The tissue concentration of salvianolic acid B was as followed in order: C_heart> C_liver>C_lung>C_intestine>C_kidney>C_spleen>C_stomach .The plasma protein binding rate of salvianolic acid B in human plasma and in rat was similar(89.2 %±1.8 %, 92.5 %±1.5 %).CONCLUSION: The method is accurate,stable and reliable,and can be used for the investigation of salvianolic acid B in pharmacokinetics research. Salvianolic acid B eliminates fast and it shows a high plasma protein binding rate,the mainly excretion way of salvianolic acid B is from biliary.

Key words: salvianolic acid B, pharmacokinetics, HPLC